Yerleşim sıklığının BALB/c ve CD-1 genotipli farelerde bazı özellikler üzerine etkisi
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2015
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Sağlık Bilimleri Enstitüsü
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This study aims to show the effects of cage density on body weight, feed comsumption, immune system, corticostrone level, adrenal gland, spleen, thymus of BALB/c ve CD-1 mice. The research has been conducted in Private Experimental Animals Laboratory, Ankara. Experiment was carried out in a Private Experimental Animals Laboratory, totally 90 BALB/c and CD-1 genotype 8 weeks of age female mice were used equally (45 mice from each genotype). The cage space per animal was arranged as control (140 cm2/2 mouse), experimental 1 (140 cm2/ 3 mouse) and experimental 2 (140 cm2/ 4 mouse) for BALB/c genotypes. This spaces were (200 cm2/2 mouse), (200 cm2/ 3 mouse) and (200 cm2/ 4 mouse) for CD-1 genotypes respectively. Body weight gain; control, experiment 1 and 2 groups of BALB/c genotype mice were 4.93; 5.30; 5.12 g, control, experiment 1 and 2 groups of CD-1 genotype mice were 4.29; 3.84; 3.64 respectively. Among CD-1 genotype density groups were a highly significant (P<0,05) differences in the 9, 10, 11, 12 weeks and the final body weight gain. For BALB/c and CD-1 genotypes mice were 5.12; 3.92 g respectively. There was no statistically significant effect of housing density or any interaction between density and genotypes, though there was a highly significant (P<0,001) differences between the two genotypes. Food comsumption; control, experiment 1 and 2 groups of BALB/c genotype mice were 317.86; 319.76; 318.96 g, control, experiment 1 and 2 groups of CD-1 genotype mice were 368.32; 362.99; 361.29 respectively. Among CD-1 genotype density groups were a highly significant (P<0,05) differences in the 9, 10, 11, 12 weeks and the final food comsumption. For BALB/c and CD-1 genotypes mice were 319.26; 364.20 g respectively. There was no statistically significant effect of housing density or any interaction between density and genotypes, though there was a highly significant (P<0,001) differences between the two genotypes. Immune levels (SRBC); control, experiment 1 and 2 groups of BALB/c genotype mice were 7.40; 7.75; 7.60, control, experiment 1 and 2 groups of CD-1 genotype mice were 7.60; 7.40; 7.20 respectively. For BALB/c genotype mice and CD-1 genotype mice were 7.58; 7.40 respectively. There was no statistically significant effect on immune levels between cage density groups and genotype groups. Corticosterone levels; control, experiment 1 and 2 groups of BALB/c genotype mice were 210.06; 192.0; 201.60, control, experiment 1 and 2 groups of CD-1 genotype mice were 218.48; 205.50; 200.76 respectively at the first day. For BALB/c and CD-1 genotype mice were 201.22; 208.25 respectively. Corticosterone levels; control, experiment 1 and 2 groups of BALB/c genotype mice were 200.20; 183.34; 191.76, control, experiment 1 and 2 groups of CD-1 genotype mice were 215.32; 208.56; 212.46 respectively at the last day. For BALB/c and CD-1 genotype mice were 191.77; 212.11 respectively. There was no statistically significant effect on corticosterone levels between cage density and genotype groups at the first and last day the experiment. Adrenal gland relative weights; control, experiment 1 and 2 groups of BALB/c genotype mice were % 0.058; 0.052; 0.054, control, experiment 1 and 2 groups of CD-1 genotype mice were % 0.040; 0.047; 0.048 respectively. For BALB/c and CD-1 genotype mice were 0.055; 0.045 respectively. There was no statistically significant effect on adrenal gland weight rate between cage density groups and genotype groups. Spleen gland relative weights; control, experiment 1 and 2 groups of BALB/c genotype mice were % 0.474; 0.486; 0.478, control, experiment 1 and 2 groups of CD-1 genotype mice were % 0.425; 0.419; 0.407 respectively. For BALB/c and CD-1 genotype mice were 0.479; 0.417 respectively. There was no statistically significant effect on spleen weight rate between cage density groups and genotype groups. Thymus gland relative weights; control, experiment 1 and 2 groups of BALB/c genotype mice were % 0.197; 0.203; 0.200, control, experiment 1 and 2 groups of CD-1 genotype mice were % 0.228; 0.211; 0.200 respectively. For BALB/c and CD-1 genotype mice were 0.200; 0.213 respectively. There was no statistically significant effect on thymus weight rate between cage density groups and genotype groups. No any injury event or aggressive behavioural properties. There was no mortality. Conclusion of study that it was observed that 30 g and over weight and 8 week age of CD-1 female mice were very longer the period of adaptation against housing density stress. The current findings indicate that 20-25 g weight and 8 week age of BALB/c female mice, housing density can be increased %50'den %100 above the current recommendation (as floor as area per mouse) in directives without no effect on experimental characteristics. Key Words: Cage Density, Corticosterone, Genotype, Immunity, Mice
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Veteriner Hekimliği