Usnik asit liken sekonder metabolitinin farklı kanser hücre tipleri üzerine etkisinin araştırılması
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Date
2016
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Biyoteknoloji Enstitüsü
Abstract
Nowadays cancer is an important public health problem and presence of undesirable side effects of cancer drugs that is often used in the treatment of cancer has stimulated the search for alternative therapeutic approaches. Therefore anticarcinogenic effects of synthetic, herbal and fungal origin drugs have been investigated against various types of cancer in recent studies. Lichens, symbiotic organisms consisting of fungi and algae and synthesize metabolites with many biological activities. As a result of research, lichens and their secondary metabolites to be used as an alternative biological organisms is suggested to be a solution for treating cancer. In the current study, screening of the anticancer effect potential of usnic acid (UA) on various types of normal (L929 and Vero) and different cancer cell line (CaCo2, HepG2, Hep2C, RD and Wehi) were investigated. The cells were treated for 24, 48 and 72h by UA at final concentration 6.25, 12.5, 25, 50, 100, 200 and 400uM. The growth inhibitory effect of UA were determined by MTT assay. Furthermore, this study was to explore mRNA expression profiles because inhibition of UA might be related to apoptotic pathway. This is the first study that explains antiproliferative effects of usnic acid on cancer and normal cells at a level of mRNA by qRT-PCR. The effect of UA on the gene expression pattern of the tumor suppressor gene TP53, Bcl and BAX- 2 were studied with qRT-PCR. Our results revealed that, 6.25 and 12.5 uM doses of UA have significant cytotoxic effect on examined cancer cell lines. Our present results suggest that usnic acid plays a major role in the regulation of apoptosis in cancer cells. The obtained finding is foreseen that lichen metabolites as UA improves cancer response to cancer treatment in patients resistant to routine chemotherapy provides supportive effect. Further study is required to enlighten additional molecular mechanisms depend on the anti-cancer activity of the lichen species and their secondary metabolites.
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