Yüksek çevre sıcaklığında barındırılan tavşanlarda bazı hematolojik ve biyokimyasal parametrelerde değişiklikler
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Date
2011
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Sağlık Bilimleri Enstitüsü
Abstract
The aim of this study is to investigate changes of some physiological, hematological and biochemical parameters in rabbits exposed to 29-30ºC ambient temperature for 72 hours.Twelve, male, healthy, 2,5-3 months aged New Zealand White Rabbits had 2,21 ± 0,47 kg weights on an average were constitued as material of study. The rabbits? adaptation were provided by keeping them at special rabbit rooms, with hepa filter tipped ventilating, which its temperature is 15-21ºC, relative humidity is 40-50 % and photoperiod is characterized by 16 hours light/8 hours dark. In this stage, the rabbits were kept in 40x50x64 cm sized cage. Commercial pellet food and water were given ad-libitum to animals.Blood samples were taken from twelve rabbits used in investigation for hematological and biochemical parameters before research. Six ones of 12 rabbits that had been taken blood samples from before research were taken to room which its temperature had been elevated to 29-30ºC, while the other 6 rabbits were being kept at room at 21ºC and they were kept in these conditions for 3 days. Before study and after adjustment of room temperatures, on days 1, 2 and 3, each rabbits? respiration rate, body temperature and live weight were measured. During 3 days, rabbits? behaviours were watched for and changes were noted. Consumed food and water amounts were determined by using digital scale and noted.On third day of research, procedure of taking blood was repeated.The blood samples which had been taken were arrived at laboratory in an hour and values of hemoglobin, hematocrit, erytrocyte, leukocyte, neutrophil, basophil, eosinophil, lymphocyte, monocyte, MCH, MCHC, MCV, thrombocyte; total protein, albumin, cholesterol, trigliseride, BUN, creatinine, glucose, AST, ALT, Na, K, Cl, Ca, P, fT3 and fT4 were determined.At the end of study, when the datas had been obtained from rabbits exposed to 29-30ºC ambient temperature for 72 hours compared to controlled group, that the rabbits located in high ambient temperature had gained less weight, had consumed less food, for all that they had consumed more water (p<0.05) were determined.An important difference wasn?t determined between values of groups and in groups in point of the respiration rates and the rectal body temperatures which noted day by day during 3 days in both groups. It was defined that the rabbits exposed to 29-30ºC ambient temperature had have less interest to environment, had been depressed and it had been easy to capture them.In rabbits exposed to 29-30ºC ambient temperature, it was determined that mean hematocrit value determined after research had decreased compared to the value determined before research. However, in values of hemoglobin, erytrocyte, leukocyte, neutrophil, basophil, eosinophil, lymphocyte, monocyte, MCH, MCHC, MCV and thrombocyte had not an important difference in both groups before and after research and between two groups (p<0.05).It was determined that serum albumin value, which had been determined before research, had decreased (p<0.05); total cholesterol and trigliseride values had increased after research in rabbits exposed to 29-30ºC ambient temperature. It was seen that the values of total protein, BUN, creatinine, glucose, AST, ALT, Na, K, Cl, Ca and P had have not a difference statistically between values of groups and in groups.Even if a significative difference between fT3 and fT4 values of two groups were not statistically determined, it was set forth that the values of fT3 and fT4 determined after research had decreased when compared to values determined before research in rabbits exposed to 29-30ºC ambient temperature.As a result, in this study, in rabbits exposed to 29-30ºC ambient temperature for 72 hours there were some changes on physiological (live weight, water and food intake), behavioral (depression), hematological (hematocrit value), biochemical (albumin, total cholesterol, trigliseride) and hormonal (fT3 and fT4) parameters, while hemoglobin, erytrocyte, leukocyte, neutrophil, basophil, eosinophil, lymphocyte; monocyte, MCH, MCHC, MCV, thrombocyte, total protein, BUN, creatinine, glucose, AST, ALT, Na, K, Cl, Ca and P were not affected by these conditions.Key Words: hematological and biochemical parameters, rabbit, 29-30ºC ambient temperature
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Keywords
tavşan, biyokimyasal parametreler