PCR detection of Mycobacterium genavense DNA in fecal samples of caged birds

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dc.contributor.authorBabacan, Orkun
dc.contributor.authorBaş, Bülent
dc.contributor.authorSareyyüpoğlu, Barış
dc.contributor.departmentVeteriner Fakültesitr_TR
dc.date.accessioned2021-10-07T14:06:48Z
dc.date.available2021-10-07T14:06:48Z
dc.date.issued2020-03-03
dc.description.abstractIn this study, pathogenic mycobacteria were investigated in fecal samples of caged birds by PCR. A total of 47 feces samples collected from 4 different aviaries in Ankara. DNA extraction from fecal samples was performed with a commercial kit using spin column technology. PCR was performed with designed primers respectively amplifying 274 base pairs (bp), 128 bp, 102 bp and 219 bp nucleotide sequences of specific genes (16SrRNA, ISI245, IS901 and hypothetical 21kDa protein gene) of Mycobacterium spp., Mycobacterium avium complex (MAC), Mycobacterium avium subsp. Avium and Mycobacterium genavense, respectively. Five samples were positive and harbored the sequence for the Mycobacterium spp., of 4 of these 5 samples was identified as M. genavense by PCR. As a conclusion of this study, which is the first announcement of the detection of M. genavense DNA in fecal samples of caged birds in Turkey, PCR was seen to be a rapid, sensitive, and a reliable method in detection of avian mycobacteriosis.tr_TR
dc.identifier.endpage204tr_TR
dc.identifier.issn/e-issn1308-2817
dc.identifier.issue2tr_TR
dc.identifier.startpage201tr_TR
dc.identifier.urihttps://doi.org/10.33988/auvfd.603515tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/75326
dc.identifier.volume67tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.33988/auvfd.603515tr_TR
dc.relation.journalAnkara Üniversitesi Veteriner Fakültesi Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectBirdtr_TR
dc.subjectFecestr_TR
dc.subjectMycobacteriosistr_TR
dc.titlePCR detection of Mycobacterium genavense DNA in fecal samples of caged birdstr_TR
dc.typeArticletr_TR

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