Ayvada (Cydonia vulgaris pers.) çenek yapraklardan somatik embriyo oluşumu ve somatik embriyoların bitkiye dönüşümü

Abstract

Immature cotyledons of open-pollinated seeds from quince (Cydonia vulgaris Pers. cv 'Limon') were excised from fruits at 7, 8, 9, 10 and 11 weeks after anthesis and cultured on Modified Murashige and Skoog medium containing Vi macroelements and iron supplemented with 250 mg/1 L-glutamine, 500 mg/1 casein hydrolysate, 0.0, 0.5, 1.0, 2.0 and 5.0 mg/1 6- benzyladenine (BA) and 0.0, 0.5, 1.0 and 2.0 mg/1 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthalene acetic acid (NAA). The cotyledons were incubated for 4 weeks in darkness, prior to subculturing on hormone-free Murashige Skoog medium containing Vi macroelements and iron supplemented with 250 mg/1 L-glutamine, 500 mg/1 casein hydrolysate in darkness for three times. Percentage of embryonic cotyledon (%) and number of somatic embryos (number per cotyledon) were determined after each subculture. In this research, the highest percentage of embryonic cotyledons (53.3-80.0%) and number of somatic embryo (1.07±0.07- 2.53±0.79) were obtained with 0.0 mg/I BA + 2.0 mg/ NAA, 0.5 mg/1 BA + 0.5 mg/1 2,4-D, 0.5 mg/I BA + 1.0 mg/1 NAA, 1.0 mg/1 BA + 2.0 mg/1 2,4-D or 1.0 mg/1 BA + 1.0 mg/1 NAA combinations in induction medium and with cotyledons cultured at 7 and 11 weeks after anthesis. Conversion of somatic embryos into plantlets was not achieved after cold treatments for 4, 8 and 12 weeks on hormone-free Murashige and Skoog medium containing % macroelements and iron.