Internal Transcribed Spacer (ITS) Fails Barcoding of the Genus Neotinea Rchb.f. (Orchidaceae)

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dc.contributor.authorHürkan, Kaan
dc.contributor.authorTaşkın, Kemal Melih
dc.contributor.departmentZiraat Fakültesitr_TR
dc.date.accessioned2021-10-01T08:16:28Z
dc.date.available2021-10-01T08:16:28Z
dc.date.issued2021-03-04
dc.description.abstractInternal Transcribed Spacer (ITS) is one of the most used barcoding regions for the molecular phylogenetics and barcoding of orchids. Our aim in this study is to test the reliability of ITS on barcoding of closely related Neotinea spp., including Neotinea tridentata, Neotinea ustulata subsp. ustulata and Neotinea ustulata subsp. aestivalis, by comparing it to the accD-psaI intergenic spacer of the plastid DNA. Both ITS and accD-psaI regions were amplified by specific primer sets and sequenced. Phylogenetic trees were regenerated by using Maximum Parsimony approach. The results showed that ITS separated some N. tridentata samples of Turkish, Greek, Hungarian and Croatian samples from the others on the phylogenetic trees due to the incomplete lineage sorting. In contrast to ITS, the accD-psaI marker could successfully separate N. tridentata and N. ustulata samples according to a priori species classification. Our findings refer to a hybridisation story between some N. tridentata and N. ustulata. We propose not to use ITS sequences directly as a barcode and to reconstruct the phylogeny of the Neotinea group. Instead, the inclusion of other nuclear regions such as LFY, ADH, etc., or utilisation of whole genome sequencing could give better barcoding results.tr_TR
dc.identifier.endpage75tr_TR
dc.identifier.issn/e-issn2148-9297
dc.identifier.issue1tr_TR
dc.identifier.startpage69tr_TR
dc.identifier.urihttps://doi.org/10.15832/ankutbd.615848tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/75104
dc.identifier.volume27tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.15832/ankutbd.615848tr_TR
dc.relation.journalTarım Bilimleri Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectDNA barcodingtr_TR
dc.subjectOrchidstr_TR
dc.subjectAccD-psaI markertr_TR
dc.titleInternal Transcribed Spacer (ITS) Fails Barcoding of the Genus Neotinea Rchb.f. (Orchidaceae)tr_TR
dc.typeArticletr_TR

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Cilt:27 Sayı:01 (2021)