Cilt:67 Sayı:04 (2020)
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Item Asymmetric conjoined twins: Gnathopagus parasiticus(Ankara Üniversitesi, 2020-09-01) Kabak, Yonca Betil; Kabak, Murat; Özak, Ahmet; Sözmen, Mahmut; İnal, Sinem; Güvenç, Tolga; Gülbahar, Mustafa Yavuz; Veteriner FakültesiIn this study, a rare case of asymmetric conjoined twins that were connected to each other by their mandibles is described. There was an amorphous mass in between mandibles of the newborn calf. Radiological examination revealed that the amorphous mass consisted of a structure similar to the mandible and skull. According to the findings, the present anomaly was asymmetric conjoined twins connected to each other by mandibles and named as gnathopagus parasiticus. Gnathopagus parasiticus case has not been reported in the literature previously.Item A case of thymic lymphoma in a rabbit(Ankara Üniversitesi, 2020-09-01) Atasever, Ayhan; Gram, Duygu Yaman; Ekebaş, Görkem; Veteriner FakültesiA five-year-old male rabbit was presented with the request of necropsy. Post mortem examination revealed a mass in the cranial mediastinum. Histopathologically, it was seen that the mass consisted of a limited mesenchymal tissue with medium-sized mature lymphocytes and a large proportion (approximately 90%) of lymphoblast-like round cell infiltration. While tumour cells were stained positively for CD3, they were negative for CD79a. Pan-Cytokeratin was localized in small number of epithelial cells in the background. Histopathological and immunohistochemical evaluation showed that the case was compatible with thymic lymphoma and it was the first case reported in rabbits in Turkey.Item The in vitro protein degradability of legume and sudan grass forage types and ensiled mixtures(Ankara Üniversitesi, 2020-09-01) STOJANOVİĆ, Bojan; ĐORĐEVİĆ, Nenad; SİMİĆ, Aleksandar; BOŽİČKOVİĆ, Aleksa; DAVİDOVİĆ, Vesna; IVETİĆ, Aleksandra; Veteriner FakültesiThis study examined the in vitro crude protein (CP) degradability of wilted, dried for hay and ensiled Medicago sativa, Trifolium pratense and Sorghum sudanense, as well as of ensiled legume-sudan grass mixtures. The rumen degradable protein (RDP) was estimated using the latest Cornell net carbohydrate and protein system (CNCPS v6.5) and with the Streptomyces griseus protease assay (48 h of incubation). Sudan grass forages were the lowest in moderately degradable CP and the highest in CP fraction C, while red clover showed the least values for the soluble true protein like alfalfa forages for slowly degradable protein bound in neutral detergent fibre (NDF). Ensiling of legume-sudan grass mixtures decreases N-NH3 content by 12.2 and 5.1% in comparison with alfalfa and red clover silages, respectively. The RDP values for sudan grass (fresh, wilted, hay) were lower by 7.2 to 7.9% or 10.4 to 15.7% (CNCPS or S. griseus procedure) and up to 0.8 or 5.3 to 9.7% in comparison with alfalfa and red clover, respectively. The reduction of protein degradability in ensiled legume-sudan grass mixtures was 5.5 or 6.1% and 1.5 or 3% compared to alfalfa and red clover silages, respectively. Due to higher rumen undegradable protein content, the sudan grass and legume-sudan grass mixtures may be efficiently used to improve the protein utilization in ruminant nutrition.Item Antibacterial activity of Turkish honey against selected foodborne pathogens(Ankara Üniversitesi, 2020-09-01) Çil, Güzin İplikçioğlu; Küplülü, Özlem; Cengiz, Görkem; Korkmaz, Seda Dicle; Arslan, Buse; Gürcan, Safa; Veteriner FakültesiHoney is an important animal product that is consumed by people of all ages and has become an important antimicrobial agent because it has both antibacterial properties and does not cause microbial resistance. Although, Turkey is among the most important honey producers of the world, there are not enough studies about the antibacterial activity of Turkish honey. According to their geographical area, honey exhibit considerable and variable antimicrobial activity. In this study, we investigated the in vitro antibacterial effect of honey obtained from Turkey, against Escherichia coli, Listeria monocytogenes, Salmonella Typhimurium and Staphylococcus aureus by using agar dilution, agar well diffusion and disc diffusion methods and compared the efficacy of these methods. Results showed the antibacterial effects of Turkish honey, collected from different regions against selected pathogens. Different concentrations of all honey samples displayed an antibacterial activity. Each microorganism exhibited different sensitivity to the honey tested. In addition, a significant difference was detected between the three methods for each microorganism and well diffusion method was found to be the most sensitive method.Item Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells(Ankara Üniversitesi, 2020-09-01) GRIGOROVA, Natalia; GÓCZA, Elen; VACHKOVA, Ekaterina; Veteriner FakültesiCardiovascular diseases are still one of the most common reasons for mortality in humans. Mesenchymal stem cells (MSCs) are preferable in cardiac regeneration cell-based therapies because of their allogeneic and high proliferative potential. The electrophysiological properties of the rabbit heard is closer to human than the mouse. The current study aimed to trace mRNA expression changes of two stemness/cardiogenic differentiation ability-related transcriptionala factors OCT4 and GATA4 in rabbit MSCs during early stages of induced cardiomyocyte differentiation in vitro. The mesenchymal stem cell originated from different anatomical areas-subcutaneous, visceral, bone marrow and pericardial tissue. The cardiac differentiation protocol for mouse embryonic stem cells in hanging drop was adopted in rabbit MSCs. The best formed embryonal bodies (EBs) like structures were collected and cultivated on gelatin-coated plates. The total mRNA was obtained before cardiac differentiation and on the 6th day after it. SYBER based real-time PCR was performed to evaluate the mRNA expression fold-changes of OCT4 and GATA4. The cultivation of MSCs in hanging drops during cardiac differentiation induced EBs formation, without any contractile activity up to the 6th day of the differentiation in all cell types. The applied differentiation protocol significantly downregulated GATA4 expression in ADSCs - EBs, while in BMSCs, both target genes were significantly upregulated. In conclusion, the adopted cardiac differentiation protocol from mouse embryonic stem cells could be a useful approach for rabbit bone marrow mesenchymal stem cells. Since the rest of the cells revealed weak cardiogenic capability at this early stage, some modifications of induction protocols should be considered.Item Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line(Ankara Üniversitesi, 2020-09-01) Melekoğlu, Abdullah; Ekici, Hüsamettin; Arat, Esra; Karahan, Siyami; Veteriner FakültesiMelamine and its metabolites pose health concern as they are used in various industrial products including feed and drugs. There are a limited number of studies on melamine and cyanuric acid cytotoxicity and cellular damage without a certain conclusion. The present study aimed to evaluate melamine, cyanuric acid and its combined cytotoxic effects using 3-(4.5-dimethylthiazol-2-yl) methyl thiazole tetrazolium (MTT) bromide test. The study also evaluated apoptotic and necrotic effect using a double staining method of Hoechst 33342 and propidium iodide. Melamine, cyanuric acid and their combination (1:1) were applied to L929 fibroblasts and Chinese hamster ovary (CHO) cells at various concentrations (1000 µg/mL, 500 µg/mL, 250 µg/mL, 125 µg/mL and 62.5 µg/mL). At the highest concentration (1000 µg/mL), the cell viability dropped down approximately to 50% both in CHO cells and L929 cells. Melamine, cyanuric acid and their mixture caused cytotoxicity in CHO cells and L929 fibroblasts in dose-dependent manner. Cell death occurred through both apoptosis and mainly necrosis. Both cell types were more sensitive to the mixture of melamine and cyanuric acid and, furthermore, CHO cells were more sensitive than L929 fibroblasts. As a result, melamine, cyanuric acid and their combination caused cytotoxicity in CHO cells and L929 fibroblasts. Further studies should be conducted in different cell lines. These studies should also aim to reveal the mechanism of cytotoxicity and related pathways.Item Potential risk in public parks: Investigation of the tick species (Acari: Ixodida) in Bursa metropolitan area, Turkey(Ankara Üniversitesi, 2020-09-01) Aydın, Levent; Girişgin, Oya; Özüiçli, Mehmet; Girişgin, Ahmet Onur; Coşkunserçe, Gözde; Veteriner FakültesiTicks feed on a wide variety of mammals, birds, reptiles, and even amphibians. The majority of ticks choose specific animal species that are of no medical or veterinary importance for feeding, while others grasp a wide range of hosts, such as pet-breeding animals or humans. They can also transmit many human and animal pathogens include viruses, bacteria, rickettsia and protozoa in Turkey. This study was carried out to detect the distribution of ticks in public parks in six districts (Osmangazi, Yıldırım, Nilüfer, Gürsu, Kestel, Mudanya) of Bursa Metropolitan Municipality area (city centre) in Turkey, between May 2016 and May 2018. A total of 6186 ticks were collected with flagging or CO2 trapping method from the public parks. The collected ticks were kept in 70% alcohol and then were identified under a stereomicroscope. Seven hard or soft tick species according to five genera were identified as Rhipicephalus sanguineus, R. turanicus, Hyalomma marginatum, H. aegyptium, Dermacentor marginatus, Ixodes ricinus and Argas persicus. Among them, R. sanguineus was the most abundant (59.79%) tick in the public parks. Ixodes ricinus increased significantly primarily in the forest areas. This is the first record of tick species in public parks in Turkey and these results may give us an acute insight into the prevalence of tick-borne infections in pets and humans.Item The relationship of fructose consumption with MDA levels in rat liver and its effect on the expression levels of COX-2 and NRF-2 genes(Ankara Üniversitesi, 2020-09-01) Özkan, Hüseyin; Kutlu, Tuncer; Veteriner FakültesiThe use of fructose as converted sugar in the food industry is quite common. Steatosis and degeneration occur due to the use of fructose in the liver where sugar is primarily metabolized. However, there is not enough information about the level of MDA and the expression levels of COX-2 and NRF-2 in the liver with low and high fructose consumption. The aim of the study is to evaluate the effect of different concentrations of fructose solutions on the expression levels of COX-2 and NRF-2 genes as well as MDA levels in the livers of rats. In this study, the effects of different fructose solution [F15; (15%) F30; (30%) and F60; (60%)] consumption for 10 weeks in rats on the MDA levels also COX-2 and NRF-2 genes expression levels in the liver were investigated. The MDA level and COX-2 gene expression level in F60 group were higher than the control and other groups (P<0.01). These findings suggest that MDA elevation in the liver may be due to inflammation. However, no change was observed in total NRF-2 gene expression. Also, a positive correlation was found between levels of COX-2 and NRF-2 expressions in F30 (0.965; P<0.01). These findings may be attributed to inflammation of liver. This may have been due to an increase in nuclear formation of NRF-2. As a result, the use of high concentration fructose solution has been found to increase the level of MDA in the liver due to inflammation.Item The qualitative and quantitative assessment of the renal cortex of the clinically healthy rabbits(Ankara Üniversitesi, 2020-09-01) Dİlek, Ömer Gürkan; Erden, Hasan; Turan, Erkut; Karakurum, Emine; STAMATOVA-YOVCHEVA, Kamelia; DIMITROV, Rosen; Veteriner FakültesiQualitative ultrasonographic images in diagnosing renal parenchymal diseases can be inadequate. The relationship between renal cortical echogenicity and parenchymal disease has been demonstrated in people. However, it is difficult to determine diffuse fat vacuoles and the parenchymal diseases at qualitative renal assessment. Disorders of the renal cortex are frequently seen in rabbits. The aim of this study was to qualitatively and quantitatively evaluate the echogenicity of the renal cortex in clinically healthy rabbits. Fourty-two clinically normal and with no history of renal disease rabbits were used. The each rabbit was investigated by complete blood count, urinalysis and renal biopsy. Renal ultrasonography was performed with a 6.5 MHz probe. For each image, three equally sized regions of interest was detected within the renal cortex. The mean pixel intensity was determined using an 8-bit grayscale, with 256 colour of grey colours. Qualitative echogenicity was evaluated by two observers. Observer 1 evaluated as an isoechoic (36/42) and hyperechoic (6/42). Observer 2 evaluated as an isoechoic (35/42) and hyperechoic (7/42). Quantitatively, the mean pixel intensities analysed for the renal cortex was 59.2±2.23 (range: 28-91). The result of this study indicates that analysis of digitized renal cortical ultrasonographic images from rabbits using the histogram technique can be used to quantitatively determine echogenicity.Item The effects of perch cooling on performance, carcass, and meat quality characteristics and behaviour of broilers reared at high temperatures with different litter thicknesses(Ankara Üniversitesi, 2020-09-01) Fidan, Evrim Dereli; Türkyılmaz, Mehmet Kenan; Nazlıgül, Ahmet; Kaya, Mehmet; Veteriner FakültesiThe aim of the present study was to investigate the effects of cooled perch and litter thickness on performance, meat quality, carcass, and behaviour traits on broilers reared at high environmental temperatures. A total of 459 1 day-old male broiler chicks were randomly assigned to nine treatment groups based on the perch treatment (no perch, non-cooled perches, cooled perches) and litter thicknesses (1, 7 and 14 cm) with three replicates. Body weight was higher in broilers reared at cooled perch group at 6 wks (P<0.05). Body weight of chickens reared under the 1 cm thickness was found higher than the 7 and 14 cm of litter thickness chickens at 6 wks. Live and carcass weights, total breast and drum weights were higher in broilers reared at 1 cm litter thickness. The perch-contact incidence of chickens reared under the cooled perch was found higher than the non-cooled chickens for all hours at 6 wks. In conclusion, it is indicated that perch cooling and 1 cm of litter thickness have a beneficial effect on broiler performance exposed to high temperatures.Item Determination of virulence factors and antimicrobial resistance of E. coli isolated from calf diarrhea, part of eastern Turkey(Ankara Üniversitesi, 2020-09-01) Cengiz, Şeyda; Adıgüzel, Mehmet Cemal; Veteriner FakültesiMicroorganisms have a primary role in the formation of calf diarrhea. Escherichia coli pose an environmental risk to young animals caused by fecal excretion. In this study, rectal swab samples (n= 133) were collected from calves with diarrhea aged from 1 day to 3 months, between August 2017 and August 2018. The samples were cultured on MacConkey agar, and then antimicrobial susceptibility and virulence genes for Escherichia coli isolates (n= 133) were investigated by disk diffusion method according to clinical and laboratory standards institute standards and multiplex polymerase chain reaction, respectively. The isolates were found to be highly resistant to oxytetracycline (78.9%), trimethoprim-sulfamethoxazole (69.2%), neomycin (60.9%), and erythromycin (58.6%). Besides, multidrug resistance was determined in 71.4% of isolates. Thirty-three of 133 (24.81%) isolates were positive for at least one virulence factor. The pathotypes of enterotoxigenic Escherichia coli (F5 and/or F41 fimbria and STa), enterohemorrhagic Escherichia coli (Stx and eae), enteropathogenic Escherichia coli (eae) and Shiga toxin-producing Escherichia coli (Stx-eae) were found in 51.5%, 6.1%, 15.2%, and 12.1%, respectively. However, the virulence properties were detected as; Stx1 (3.03%), Stx2 (9.09%), STa (21.21%), and eae (15.15%); the F41 and F5 were not detected. Also, the fifteen-point two percent of strains (5/33) were the hybrid type that carried both Stx (either Stx1 or Stx2) and enterotoxigenic Escherichia coli specific enterotoxin gene STa. The existence of different virulence factors found in this study supports the statement that calves are possible bearers of pathogens that are dangerous to public health.Item The effects of thyme essential oil and vitamin combinations on performance, carcass quality and oxidation parameters in broilers exposed to heat stress(Ankara Üniversitesi, 2020-09-01) Sarıözkan, Savaş; Güçlü, Berrin Kocaoğlu; Konca, Yusuf; Aktuğ, Eray; Kaliber, Mahmut; Beyzi, Selma Büyükkılıç; Şentürk, Meryem; Veteriner FakültesiThis study aims to determine the effect of thyme essential oil (TEO) and a combination of TEOwith different vitamins (A, C and E) on performance, carcass quality, oxidation parametersin broilers exposed to heat stress (HS). A total of 300, 21 days old, male Ross-308 chicks were distributed in six experimental groups with five replicates. The broilers in HS groups were exposed to 34oC for nine hours per day between 21 and 41 days of the experiment. The experimental groups were as follows: 1: Thermoneutral control (TNC, 22oC), 2: Heat stress control (HSC, 34oC), 3: TEO(300 mg/kg TEO, 34oC), 4: TEO+ Vit C (300 mg/kg TEO+ 250 mg/kg Vit C, 34oC), 5: TEO+ Vit E (300 mg / kg TEO+ 250 mg/kg Vit E, 34oC) and 6: TEO+ Vit A (300 mg / kg TEO+ 15000 IU / kg Vit A, 34°C). As a result, it was determined that HS significantly reduced live weight (LW), live weight gain (LWG), and feed intake(FI) values compared to the TNC group (P<0.05). Supplementation of TEOor TEOand vitamin (A, C, E) combinations to the feed of broilers exposed to HS had no significant effect on performance, carcass quality, carcass part yields, and serum parameters. Economically, the highest profitability was obtained in the TNC group (P<0.001), In conclusion, HS negativelyaffected the performance of broilers, and the supplementation of TEO+ Vit E to the diet had slightly reduced the adverse effects of HS.Item Determination of Vibrio parahaemolyticus in seafoods using direct plate counting, quantitative loop-mediated isothermal amplification and propidium monoazide-qLAMP(Ankara Üniversitesi, 2020-09-01) Doğruer, Yusuf; Telli, A. Ezgi; Dil ve Tarih-Coğrafya FakültesiOne of the most challenging aspects in culture independent methods for foodborne pathogens’ detection is discrimination of dead and live microorganisms. This study aimed to determine the Vibrio parahaemolyticus in seafoods via direct plate counting (DPC) and toxR-based quantitative loop-mediated isothermal amplification (qLAMP) and to discriminate dead and live cells using propidium monoazide (PMA)-qLAMP. A total of 200 samples including finfishes (n= 100) and shrimps (n= 100), representing the Mediterranean, Black and Aegean sea were collected from supermarkets and fish markets of Konya-Turkey. qLAMP was performed in a Real-Time Turbidimeter and the time threshold (tt) values were yielded in 60 minutes. On DPC, the colonies grown on TCBS Agar was further confirmed by conventional PCR based from gyrB1 gene of Vibrio spp. and toxR gene of V. parahaemolyticus. Virulence property of the isolates were determined by tdh based qLAMP. The detection limit of the qLAMP was 1.2×104 CFU/g in artificially contaminated seafoods. DPC, qLAMP and PMA-qLAMP detected V. parahaemolyticus in 8 (4%), 12 (6%) and 12 (6%) samples, respectively. The CFUs of V. parahaemolyticus detected in qLAMP (5.96±0.10 log10 CFU/ml) and PMA-qLAMP (4.71±0.13 log10 CFU/ml) were higher than those of DPC (1.99±0.44 log10 CFU/ml) (P<0.05). The mean tt reduction in PMA treated samples was 1.25±0.38 log10 CFU/sample. The tdh gene was not detected in any of the isolates. In conclusion, the toxR-based PMA-qLAMP method could be an alternative to be used more widely and effective assay for the quantification of live V. parahaemolyticus in seafoods.Item Histidine and tyrosine decarboxylase activities of lactic acid bacteria in sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax)(Ankara Üniversitesi, 2020-09-01) Doğan, Yağmur Nil; Pamuk, Şebnem; Gürler, Zeki; Veteriner FakültesiBiogenic amines (BAs) are formed by decarboxylation of amino acids, amination and transamination of aldehyde and ketone groups. The excess of BAs is harmful to human health. BAs play a significant role in determining the shelf life and quality of foods. Released type and amount of BAs depend on factors such as the quality of the raw material, the diversity of natural microbiota, processing and storage conditions. In fish, the release of BAs is affected primarily from microbial growth as well as other reasons and may cause poisoning. It was aimed to determine the possibility of histidine decarboxylase activity gene (hdc) and tyrosine decarbocylase activity gene (tyrdc) in lactic acid bacteria (LAB) which were isolated from sea bream and sea bass. A total of 18 Gram positive-catalase negative LAB was isolated from 84 fish samples from 14 different fish markets. It was found that 12 out of 18 LAB (67%) isolates showed negative histidine and tyrosine decarboxylase activities. While 2 out of 6 (11%) LAB isolates were determined positive only tyrosine decarboxylase and 4 of them (22%) were positive for histidine and tyrosine decarboxylase. As a result of the Polymerase Chain Reaction (PCR), 9 out of 12 LAB isolates (75%) were found to have histidine decarboxylase activity gene. As a result, the prevalence of histidine decarboxylase activity gene in the LAB has detected more extensive than tyrosine decarboxylase activity gene. Increasing the studies examining the presence of aminobiogenic microorganisms in fish is important for the protection of public health.Item Some production characteristics of Bafra, Akkaraman, Bafra × Akkaraman F1 and B1 sheep genotypes(Ankara Üniversitesi, 2020-09-01) Güngör, Ömer Faruk; Ünal, Necmettin; Veteriner FakültesiThe present study was carried out to identify the reproductive characteristics, survival rate, growth traits and some body measurements of Bafra (B), Akkaraman (A), Bafra × Akkaraman F1 (BAF1) and Bafra × Akkaraman B1 (BAB1) sheep genotypes. The research was performed at Gözlü State Farm, belonging to the General Directorate of Agricultural Enterprise (TİGEM), during the years of 2014-2016. In the study, lambing rates of B×B, A×A, B×A and B×BAF1 mating groups were 55.25, 69.97, 67.25 and 64.91%; the lamb production were 92.27, 88.93, 87.54 and 122.81% and the litter sizes were 1.67, 1.27, 1.30 and 1.89, respectively. The survival rates of B, A, BAF1 and BAB1 lambs were calculated as 89.89, 94.23, 88.79 and 98.36% in the 90th day, respectively (P<0.05). The least square means of these lambs were 3.65±0.05, 4.71±0.07, 4.56±0.06 and 4.03±0.10 kg (P<0.001) for birth weight and 23.51±0.30, 31.39±0.37, 30.00±0.35 and 25.16±0.53 kg (P<0.001) for the 90th day weight (weaning weight), respectively. Body measurements of these genotypes on the 90th day were 56.79±0.45, 61.75±0.54, 60.98±0.59 and 61.73±0.62 cm (P<0.001) for the withers height; 55.76±0.32, 55.18±0.39, 56.91±0.42 and 57.43±0.45 cm (P<0.01) for the body length; 63.05±0.73, 71.88±0.89, 60.89±0.96 and 61.92±1.02 cm (P<0.001) for the chest girth; and 16.63±0.50, 40.45±0.62, 28.33±0.67 and 18.33±0.70 cm (P<0.001) for the widest tail circumference, respectively. In conclusion, the reproductive performance of the BAF1 genotype was satisfactory; BAF1 lambs were partially similar to A lambs, but BAB1 lambs were partially similar to B lambs in terms of growth characteristics.