Cilt:31 Sayı:01 (2022)
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Item Contributions to the flora of Beyağaç (Denizli)(Ankara Üniversitesi Fen Fakültesi, 2022) Çiçek, Mehmet; Other; OtherIn this study, in order to determine the flora of Beyağaç (Denizli), 767 plant samples were collected and observed from 74 different localities by the field studies carried out in Beyağaç and its close surroundings in the years 2017 and 2018. The results of examination of the collected plant samples and evaluation of the observed plants, it was determined 362 species and totally 363 plant taxa, belonging to 63 families and 228 genera. Of 363 vascular plant taxa, 3 taxa belong to Pteridophyta division, 360 taxa to Magnoliophyta division. Of the Magnoliophyta division, 4 taxa belong to Gymnospermae subdivision and 356 taxa to Angiospermae subdivision. Of the Angiospermae subdivision, 312 taxa belong to Dicotyledonae classis and 44 taxa to Monocotyledonae classis. The phytogeographical spectrum of the flora of Beyağaç (Denizli) as follows: Mediterranean elements 115 taxa (31.6%), Irano-Turanian elements 13 taxa (3.6%), Euro-Siberian elements 20 taxa (5.5%) and multi-regional or unknown origin 216 taxa (59.3%). The largest ten families in flora of Beyağaç (Denizli) as follows: Fabaceae 43 taxa (11.8%), Asteraceae 42 taxa (11.5%), Lamiaceae 28 taxa (7.7%), Caryophyllaceae 27 taxa (% 7.4), Brassicaceae 24 taxa (6.6%), Poaceae 16 taxa (4.4%), Boraginaceae 14 taxa (3.8%), Apiaceae 13 taxa (3.6%), Asparagaceae 11 taxa (3.0%), and Plantaginaceae 10 taxa (2.7%). The largest ten families constitute 64% of the flora of Beyağaç (Denizli). The largest ten genera in the flora of Beyağaç (Denizli) as follows: Alyssum L. 8 taxa (2.2%), Silene L. 7 taxa (1.9%), Medicago L. 6 taxa (1.6%), Salvia L. 6 taxa (1.6%), Cerastium L. 5 taxa (1.4%), Euphorbia L. 5 taxa (1.4%), Muscari Mill. 5 taxa (1.4%), Teucrium L. 5 taxa (1.4%), Veronica L. 5 taxa (1.4%), and Vicia L. 5 taxa (1.4%). The number of endemic taxa in the flora of Beyağaç (Denizli) are 39 (10.7%). Their threat categories were given according to “Red Data Book of Turkish Plants”. It is found 21 taxa in LC category, 4 taxa in NT category, 5 taxa in VU category, 6 taxa in EN category and 2 taxa in CR category.Item Ankara Üniversitesi Fen Fakültesi(Ankara Üniversitesi Fen Fakütesi, 2022) Atasagun, Sibel; Biyoloji; Fen FakültesiLemna minor L. has been traditionally used for a long time for its analgesic, antipyretic, vitamin C supplement, astringent, antipruritic effects. Although there are many heavy metal removals using L. minor, unfortunately, biological activity studies are very limited. In this study, the chemical content and total phenol content, DPPH removal, metal chelation (Fe2+) and β-Carotene-lycopene methods of the L. minor macrophyte we obtained from Turkey were determined by GC-MS. The results of the study showed that our plant contains 25 different essential oils and has a high phenol content. In addition, 72% DPPH removal of L.minor was determined when it had 71% iron chelating ability. As a result of our study, it has been revealed that the L. minor we use is an effective antioxidant. It is thought that its usability in the fields of food and medicine can be investigated with further studies.Item An investigation of Rabies virus existence on rodents by using new PCR primer pairs(Ankara Üniversitesi Fen fakültesi, 2022) Günkan, Tugce; Other; OtherZoonotic diseases are the infections to be carried between human and other vertebrates. Rhabdoviruses belong to a virus family which could infect a wide range of host organism. It is important to find new molecular diagnostic tools and primers for the identification of the viruses to be able to make the molecular identification process faster and reveal new forms of the virus. Rodents are the primary mammals group that can uncontrollably go in and out from quarantine regions. Therefore, in this study, 242 Apodemus spp. (wood mouse) and Myodes glareolus (bank vole) specimens collected from 16 localities in province Zonguldak, Çaycuma district were used to scan brain tissues to determine RABV using hemi-nested PCR. Also, to determine RABV a new primer pairs were designed using already published sequences. According to results, eight specimens were showed positive bands for RABV. Those eight sequences blasted. But the sequences did not match according to the Blast result. The designed primer pairs provide positive bands on electrophoresis for positive control so that the primer pairs are new and can be used for following studies. With this study it was also tested whether rodents are the potential carriers for RABV since they are primarily prey source for carnivores, and domestic animals.Item Antioxidant activities of Turkish extra virgin olive oils(Ankara Üniversitesi Fen Fakültesi, 2022) Anar, Okan; Biyoloji; Fen FakültesiExtra virgin olive oil is the highest grade of virgin olive oil derived by cold mechanical extraction without the use of solvents or refining methods. These olive oils are known for their composition in phenolic compounds that have antioxidant properties. This study aims to determine the total phenolic and flavonoid compounds and antioxidant activities of four Turkish extra virgin olive oil samples: Kilis yağlık, İzmir sofralık, Ayvalık, and Tavşan yüreği. The highest sample concentration used for the experiments was 4 mg/ml while 1 mg/mL was used for ABTS radical scavenging assay. The lowest total phenolic and flavonoid content was observed in Tavşan yüreği sample. All extra virgin olive oil samples showed scavenging activity against DPPH and ABTS free radicals. Extra virgin olive oil samples with high phenolic and flavonoid content presented more effective radical scavenging activity with low IC50 values. This study provides information about the phenolic content and antioxidant activities of four important Turkish olive oil samples.Item Detailed characterization of lectin genes in common bean using bioinformatic tools(Ankara Üniversitesi Fen Fakültesi, 2022) Aras, Sümer; Biyoloji; Fen FakültesiBecause of differences in molecular structure, biochemical properties, and carbohydrate binding specificity, lectins are considered a complex and heterogeneous group of proteins found in all organisms. Plant lectins are important proteins in terms of their benefits in cancer treatments, biomedical applications, and many medical uses due to their numerous biological roles such as intercellular interactions, defense mechanisms formation, immunomodulation, and anticarcinogenic activity. Despite the discovery of significant amounts of lectin proteins in different plant species, many questions about their potential biological role remain unanswered in P. vulgaris L. In this study, using bioinformatics tools, 52 Pvul-LEC genes were identified in the P. vulgaris genome and these genes were clustered into three subgroups based on phylogenetic analysis. The majority of Pvul-LEC proteins in the same subfamily of phylogenetic tree shared similar motifs and gene structures. Eight pairs of segmental duplications were discovered based on genome wide duplication analysis. Pvul-LEC proteins' three-dimensional structure and functions were also predicted. Simultaneously, gene expression levels of Pvul-LEC genes against drought and salt stress in leaf tissues were evaluated based on publicly available RNAseq data. As a result, it is anticipated that the data obtained in the current study will be beneficial to literature and following studies related to lectin genes.