Cilt:68 Sayı:01 (2021)

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https://dspace.ankara.edu.tr/handle/20.500.12575/75360

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Browsing Cilt:68 Sayı:01 (2021) by Subject "Embryo"

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    Effects of genotype on the biomechanical parameters and composition of bone in the laying hen embryos
    (Ankara Üniversitesi, 2020-12-25) Elibol, Fatma Kübra Erbay; Onbaşılar, E. Ebru; Karakan, Tuba; Taban, Süleyman; Demir, Teyfik; Veteriner Fakültesi
    Bone problems are highly prevalent in laying hens. These problems affect the welfare, production and economic losses. Bone development begins in the embryonic period, and if the skeletal system develops well at that time, the subsequent production period can be affected positively. The present experiment aimed to investigate the effect of genotype on biomechanical parameters and composition of bone in the laying hen embryos. For this purpose, 360 fertilized eggs were obtained from two brown (Atak-S and Brown Nick) and two white (Atabey and Nick) layer breeders and incubated. Metatarsus, tibia and femur properties were examined on the embryonic d 19 and 21. Results showed that genotype played an important role in determining the biomechanical properties and mineral composition of the metatarsus, tibia and femur in the embryonic period. Examined bone characteristics improved with embryonic age. The least mineralization was observed in the metatarsus bone. In conclusion, bone properties were influenced from the genotype. However these differences were not related with laying hens being white or brown. The effect of the interaction between genotype and embryonic age on the bone properties should be considered.
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    Improvement of bovine in vitro embryo production by fetal calf serum and cysteamine supplementation and investigation of freezability
    (Ankara Üniversitesi, 2020-12-25) Sandal, Asiye İzem; Elgün, Tuba; Şenlikçi, Hatice; Veteriner Fakültesi
    The aim of this study was to investigate the effects of Cysteamine (Cys) and Fetal Calf Serum (FCS) in Synthetic Oviduct Fluid (SOF) and Charles and Rosenkrans (CR1aa) on the bovine in vitro embryo production and its ability of freeze. The oocytes were divided into two groups and allowed to mature in TCM-199, with and without cysteamine. They were divided into 4 subgroups according to whether they contain Cys and FCS in 2 different culture media (SOF and CR1aa). Accordingly, 8 groups were formed as SOF+FCS+Cys, SOF+Cys, SOF+FCS, S Dulbeco's OF, CR1aa+FCS+Cys, CR1aa+Cys, CR1aa+FCS and CR1aa. Embryos were cultured for 7-9 days and were frozen using vitrification method. Development of embryos was observed during the first 24 hr post-thaw period. The highest rate of compact morula was 20% in SOF+FCS+Cys and 19.4% in CR1aa+FCS+Cys. The rate of blastocyst in SOF+FCS and CR1aa+FCS were found 17.9% and 15.4% respectively and the difference between groups was statistically significant (P<0.05). During the 12 hr vitality assessment, the highest rate was determined in SOF+FCS (47%). The results indicate that FCS has a positive effect in reaching compact morula and blastocyst regardless of the presence of the culture medium or antioxidant used. There was no statistically difference between the values of post-thaw embryos.