Cilt:68 Sayı:01 (2021)

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https://dspace.ankara.edu.tr/handle/20.500.12575/75360

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Effects of epidural bupivacaine, bupivacaine-butorphanol and bupivacaine-morphine on postoperative analgesia for hind limb and pelvic orthopedic operations in dogs
(Ankara Üniversitesi, 2020-12-25) Karslı, Birkan; Pekcan, Zeynep; Kumandaş, Ali; Kürüm, Barış; Çınar, Miyase; Veteriner Fakültesi
The aim of this study was to compare the postoperative analgesic effects of epidural bupivacaine, bupivacaine-butorphanol and bupivacaine-morphine in hind limb and pelvic orthopedic operations in dogs. Three groups each containing 10 dogs were formed. Through the lumbosacral space, group B was administered bupivacaine, 1 mg kg-1; group BB was administered bupivacaine, 1 mg kg-1 and butorphanol, 0.25 mg kg-1; and group BM was administered bupivacaine, 1 mg kg-1 and morphine, 0.1 mg kg-1. Anaesthesia was induced with diazepam (0.2 mg kg-1) and propofol (5 mg kg-1) and maintenance with isoflurane in oxygen. Pain assessment was performed with the University of Melbourne Pain Scale (UMPS) by a blinded observer. Plasma cortisol levels were measured preoperatively and postoperatively. Postoperative pain scores were significantly lower at 2 hours in the group BM, and at 8 and 12 hours in the groups BB and BM compared to the group B (P=0.007). Group BM had a lower plasma cortisol level than the group B at 2 hours (P=0.002). Bupivacaine-butorphanol and bupivacaine-morphine use were shown to provide longer analgesia in the postoperative period; and the epidural administration of bupivacaine-butorphanol and bupivacaine-morphine was considered to be potentially useful in clinical practice.
Clinicopathologic evaluation of oral squamous cell carcinoma in a young dog
(Ankara Üniversitesi, 2020-12-25) Gürgen, Hazal Öztürk; Egeden, Evrim; Şennazlı, Gülbin; Veteriner Fakültesi
Canine oral papillomas is a benign tumor of young dogs and caused by papillomavirus. The possible role of papillomavirus infection in the development of oral squamous cell carcinoma has recently been studied, but it has not been elucidated in veterinary medicine yet. One-year-old, mixed, spayed, a female dog was presented with severely disseminated oral lesions, lethargy, and weight loss. Physical examination of the patient revealed severely disseminated oral papillomatous lesions in the entire oral cavity and the complete blood test showed mild non-regenerative anemia and pancytopenia. In addition, the patient was found seropositive by the SNAP 4Dx Plus test for Ehrlichia canis. Histopathologic examination of oral lesions was performed using Hematoxylin and Eosin (HE) staining and immunohistochemistry for p16 antibody which increases in infections caused by papillomavirus. Histopathology revealed the histologic features of oral papilloma in association with squamous cell carcinoma. Cytoplasmic and nuclear positive reactions for p16 protein were observed within the neoplastic cells in the immunohistochemical examination. Thereafter, the dog was treated with combined therapy of vincristine, antibiotic, radiotherapy, and high doses of vitamin C. After long-term treatment, the dog completely recovered from the lesions. In this report, it was aimed to present a possible role of papilloma in the development of oral squamous cell carcinoma with the clinical, histopathological, immunohistochemical findings and treatment procedure.
Molecular presence of Felis catus gammaherpesvirus -1 in cats with ocular disorders in Turkey
(Ankara Üniversitesi, 2020-12-25) Koç, Bahattin Taylan; Bircan, Müge; Veteriner Fakültesi
Felis catus gammaherpesvirus-1 (FcaGHV-1) is a newly discovered feline virus, and its effects on the health of cats are not certainly known. Known feline viruses act a huge role in ocular disorders in domestic and wild cats. Also, emerging viruses may be responsible for ophthalmic diseases of cats. We aimed to investigate the presence of FcaGHV-1, Feline calicivirus (FCV), Feline herpesvirus -1 (FHV-1), Feline immunodeficiency virus (FIV), Feline infectious peritonitis virus (FIPV), Feline leukemia virus (FeLV), Feline panleukopenia virus (FPV) in cats in Turkey by molecular methods, and to examine the relationship between the virus and ocular disorders by obtained data. Swab, blood and biopsy samples were collected from 45 owned cats with ocular disorders. Molecular techniques including viral genome amplification and sequencing were used in this study. Both blood-swabs and biopsy samples were in two out of 45 cats (2/45; 4.4%) found to be FcaGHV-1 and FIV positive. Our two sequences and a reference sequence from Japan (LC437925) have constructed a separated subgroup in the molecular phylogenetic tree. In this study, we have not found a tight relationship between ocular disorders and FcaGHV-1. This is the first study exhibiting the presence of FcaGHV-1 and its molecular status in Turkey. Acquired knowledge suggests performing further studies on FcaGHV-1.
Effects of genotype on the biomechanical parameters and composition of bone in the laying hen embryos
(Ankara Üniversitesi, 2020-12-25) Elibol, Fatma Kübra Erbay; Onbaşılar, E. Ebru; Karakan, Tuba; Taban, Süleyman; Demir, Teyfik; Veteriner Fakültesi
Bone problems are highly prevalent in laying hens. These problems affect the welfare, production and economic losses. Bone development begins in the embryonic period, and if the skeletal system develops well at that time, the subsequent production period can be affected positively. The present experiment aimed to investigate the effect of genotype on biomechanical parameters and composition of bone in the laying hen embryos. For this purpose, 360 fertilized eggs were obtained from two brown (Atak-S and Brown Nick) and two white (Atabey and Nick) layer breeders and incubated. Metatarsus, tibia and femur properties were examined on the embryonic d 19 and 21. Results showed that genotype played an important role in determining the biomechanical properties and mineral composition of the metatarsus, tibia and femur in the embryonic period. Examined bone characteristics improved with embryonic age. The least mineralization was observed in the metatarsus bone. In conclusion, bone properties were influenced from the genotype. However these differences were not related with laying hens being white or brown. The effect of the interaction between genotype and embryonic age on the bone properties should be considered.
Detection of SARS-CoV-2 using five primer sets
(Ankara Üniversitesi, 2020-12-25) Karagöz, Alper; Tutun, Hidayet; Arslantaş, Tutku; Altıntaş, Özlem; Koçak, Nadir; Altıntaş, Nadir; Veteriner Fakültesi
A novel coronavirus (SARS-CoV-2) outbreak, responsible for a pneumonia-associated respiratory disorder (COVID-19), has started in early December 2019 in Wuhan, China, and has rapidly spread around the world. Rapid and accurate diagnostic testing plays a crucial role in tackling the COVID-19 pandemic. In this study, it was aimed to compare 5 primer sets designed to amplify different regions for the detection of SARS-CoV-2 and to perform sequence analysis. Conventional RT-PCR was carried out using primers targeting different regions of the virus genome including ORF1ab, Envelope (E), RNA-dependent RNA polymerase (RdRp), Spike (S) and Nucleocapsid (N) genes for the diagnosis of COVID-19. DNA sequence of ORF1ab gene from each sample were compared with the DNA sequence data of SARS-CoV-2 stored in the GenBank and ORF1ab phylogenetic tree was constructed. The amplicon sizes of ORF1ab, S, E, N and RdRp genes were 588 bp, 440 bp, 145 bp, 323 bp and 196 bp, respectively. The SARS-CoV-2 RNA was detected from 74% of total samples from RdRp gene, 87% for N gene, 74% for S gene, 61% for E gene and 82% for ORF1ab region. The ORF1ab sequences of SARS-CoV-2 from 82 patients were had 100% identity to the sequence of Wuhan isolate and among themselves. The phylogenetic analysis revealed that all isolates formed a cluster. The results of this study suggest that the N region is the best for SARS-CoV-2 identification.
An investigation on the renal portal system in long-legged buzzard (Buteo rufinus)
(Ankara Üniversitesi, 2020-12-25) Sizer, Sedef Selviler; Kabak, Murat; Onuk, Burcu; Veteriner Fakültesi
This study was carried out for the morphological examination of renal portal system in the long-legged buzzard. A total of nine adult long-legged buzzards were used in the study. The latex procedure was performed for the macroanatomic detection of the vessels forming the renal portal system. Dissections were performed under the stereomicroscope and later photographs were taken. The renal portal system vessels formed by the cranial renal portal vein and caudal renal portal vein in the long-legged buzzard. Afferent renal branches were separated from these vessels and efferent renal branches were observed to join renal veins. The anastomosis of the left cranial renal portal vein and right cranial renal portal vein was not observed at the cranial side, besides the presence of interiliac anastomosis was determined between the right internal iliac vein and left internal iliac vein at the caudal side. The findings of the renal portal system of long-legged buzzard (Buteo rufinus) were generally similar to those noted in other birds.
Improvement of bovine in vitro embryo production by fetal calf serum and cysteamine supplementation and investigation of freezability
(Ankara Üniversitesi, 2020-12-25) Sandal, Asiye İzem; Elgün, Tuba; Şenlikçi, Hatice; Veteriner Fakültesi
The aim of this study was to investigate the effects of Cysteamine (Cys) and Fetal Calf Serum (FCS) in Synthetic Oviduct Fluid (SOF) and Charles and Rosenkrans (CR1aa) on the bovine in vitro embryo production and its ability of freeze. The oocytes were divided into two groups and allowed to mature in TCM-199, with and without cysteamine. They were divided into 4 subgroups according to whether they contain Cys and FCS in 2 different culture media (SOF and CR1aa). Accordingly, 8 groups were formed as SOF+FCS+Cys, SOF+Cys, SOF+FCS, S Dulbeco's OF, CR1aa+FCS+Cys, CR1aa+Cys, CR1aa+FCS and CR1aa. Embryos were cultured for 7-9 days and were frozen using vitrification method. Development of embryos was observed during the first 24 hr post-thaw period. The highest rate of compact morula was 20% in SOF+FCS+Cys and 19.4% in CR1aa+FCS+Cys. The rate of blastocyst in SOF+FCS and CR1aa+FCS were found 17.9% and 15.4% respectively and the difference between groups was statistically significant (P<0.05). During the 12 hr vitality assessment, the highest rate was determined in SOF+FCS (47%). The results indicate that FCS has a positive effect in reaching compact morula and blastocyst regardless of the presence of the culture medium or antioxidant used. There was no statistically difference between the values of post-thaw embryos.
Treatment of dermatophilosis with oxytetracycline and tylosin combination in Saanen goat kids
(Ankara Üniversitesi, 2020-12-25) Aydoğdu, Uğur; Baydar, Ersoy; Usta, Mustafa; Dokuzeylül, Banu; Karaman, Musa; İlhan, Ziya; Veteriner Fakültesi
In this study, it was aimed to determine the treatment efficacy of oxytetracycline and tylosin combination in Saanen goat kids with Dermatophilus congolensis infection. Samples, were collected from the 27 of 4-5-month-old Saanen goat kids, and analyzed from bacteriological, mycological and ectoparasitological aspects. D. congolensis was isolated from the samples as pure culture. In the mycological analysis of the samples, no microorganisms were found, while flea was detected in ectoparasitological examinations. A combination of oxytetracycline and tylosin was recommended for dermatophilosis treatment, and the lesions regressed 5 days after the treatment and disappeared over time. It was concluded that D. congolensis infection in Saanen goats kids were observed for the first time in Turkey and can be treated successfully with oxytetracycline and tylosin combination.
Effect of adding humate to the ration of dairy cows on yield performance
(Ankara Üniversitesi, 2020-12-25) Yüca, Songül; Gül, Mehmet; Veteriner Fakültesi
The aim of this study is to investigate the effects of different levels of humate addition to colostrum quality, milk composition, somatic cell count, some blood metabolites and reproductive performance in the period from prepartum 40th to postpartum 60th day. In this study, dairy cows from prepartum 40th to postpartum 60th day were given humate additive which was according to dosed on dry matter consumption basis from (75 g, 150 g). In the study, 26 Swiss Brown cows in the same care and feeding conditions were used in the second lactation. The feeds were weighed to determine the dry matter intake of the individual cows daily. Blood was collected from the vena jugularis at 40, 30, 20 and 10 days before the estimated date of birth, at calving (day 0) and on days 5, 10, 20, 30, 45, and 60 after birth. Colostrum and milk samples were taken in the study. As a result of the study, it has been determined that the use of humate additives has no effect on body condition score, body weight, in milk non-fat milk solid, density, protein, lactose, freezing point, somatic cell count, in blood triglicerides, phosphorus, magnesium, albumin, glucose, blood urea nitrogen. It was found that with the addition of humate additive to the feed, colostrum specific gravity increased, prepartum and postpartum dry matter intake increased, milk yield and the fat percentage increased, serum non-esterified fatty acid and blood beta-hydroxybutyric acid levels decreased, and postpartum serum calcium level increased. It was concluded that 75 g of humate, which is determined based on dry matter consumption, can be added to the rations of the dairy cows at this dose and has positive effects on colostrum quality, milk yield, milk fat ratio, and negative energy balance.
Characterization of Pasteurella multocida isolates recovered from the oral flora of cats
(Ankara Üniversitesi, 2020-12-25) Tınmaz, Tuğçe; Çelik, Baran; Halaç, Baran; Bağcıgil, Arzu Funda; Veteriner Fakültesi
In this study, it was aimed to investigate the presence of Pasteurella multocida in the swab samples taken from the oral cavity of cats, and to determine the capsular type and antimicrobial susceptibility of the isolates. For this purpose, swab samples taken from 300 cats were inoculated onto Knight's selective enriched medium and blood agar for isolation of P. multocida. Following after capsular typing of the isolates by PCR, the susceptibilities of the isolates to ceftiofur, clindamycin, erythromycin, enrofloxacin, amoxicillin+clavulanic acid, trimethoprim/ sulfamethoxazole, tetracycline, and chloramphenicol were examined by the disk diffusion method. The relationships between oral colonization and various physiological and behavioral variables were evaluated, statistically. P. multocida was isolated from 48 (16%) of the samples and all strains were typed as capsular type A. While all of the isolates were resistant to clindamycin and susceptible to enrofloxacin, 8,33% tetracycline resistance was also remarkable. Multiple antibiotic resistance (MDR) was detected in 27% of the isolates. The colonization of P. multocida was found to be statistically significant in cats under the age of 1 and in cats living outdoors. The importance of antibiotic resistance observed in bacteria with a zoonotic character such as P. multocida should not be ignored since it poses a threat to public health. It was concluded that, in this study determination of tetracycline resistance, which has started to be reported in feline Pasterurella multocida isolates in recent years, and detection of multiple antibiotic resistance in 27% of isolates, was extremely important for public health.
Priapism and its surgical treatment in a cat
(Ankara Üniversitesi, 2020-12-25) Sarıtaş, Zülfikar; Korkmaz, Musa; Görücü, Fatma; Çelik, Sefa; Veteriner Fakültesi
This study details a case of priapism in a 3-year-old, male, crossbreed cat weighing 5,4 kg treated successfully with surgery. The cat was brought to the Afyon Kocatepe University Veterinary Health Application and Research Centre for general examination and castration. Clinical examination revealed a fully erect and hard/solid penis. Serum biochemistry, complete blood count, and testosterone results of the animal were within the reference ranges except for urea. When the conservative treatment was unsuccessful, it was decided to perform a perineal urethrostomy operation. Postoperative examination on day 10 revealed healing and comfortable urination; thus, the stitches and urinary catheter were removed. In conclusion, this report of successful treatment of priapism in a cat through perineal urethrostomy can contribute to veterinary literature.
Slaughter and carcass characteristics of Kivircik lambs in different rearing seasons
(Ankara Üniversitesi, 2020-12-25) Yalçıntan, Hülya; Öztürk, Nurşen; Akın, Pembe Dilara; Ekiz, Bülent; Koçak, Ömer; Yılmaz, Alper; Veteriner Fakültesi
The aim of this study was to determine the slaughter and carcass characteristics of Kivircik lambs reared in different seasons. A Total of 36 Kivircik male lambs, which were reared in different seasons, were used in the study; autumn rearing (AR, n= 12 lambs), spring-summer rearing (SSR, n= 12 lambs), winter rearing (WR, n= 12 lambs). All the lambs in a specific rearing season were slaughtered at approximately 134 days old. Pre-slaughter live weight, hot carcass weight and real dressing percentage for WR, SSR and AR groups were 27.97, 20.65, and 21.70 kg; 12.94, 7.83, and 8.87 kg, and 55.15, 50.25, and 50.05%, respectively (P < 0.001). WR lambs had significantly higher carcass and hind limb compactness score as well as ribs percentage than SSR and AR lambs (P < 0.001). The results indicated that lambs from WR group had better carcass characteristics than lambs from AR and SSR groups.
Long-term prospective assessment of subconjunctival triamcinolone acetonide in addition to topical therapy in the management of chronic superficial keratitis
(Ankara Üniversitesi, 2020-12-25) BETEG, Florin; LELESCU, Cristina; URDĂ-CÎMPEAN, Andrada; TAULESCU, Marian; MURESAN, Cosmin; Veteriner Fakültesi
Chronic superficial keratitis (CSK) is a progressive inflammatory disease in adult dogs, associated with corneal vascular proliferation, edema and melanic pigment deposition. In the absence of a curative therapy, the current approach for the management of CSK aims to control disease progression and eventually decrease the severity of corneal lesions. Subconjunctival injections of corticosteroids may be effective in severe cases, where topical therapy alone is inadequate. The aim of this study was to comprehensively evaluate the effectiveness of subconjunctival triamcinolone acetonide (TA) injection in addition to topical steroid treatment for the long-term management of CSK, in dogs unresponsive to conventional topical steroids. Treatment efficacy was assessed in 11 dogs with bilateral CSK by periodic evaluation of the cornea in terms of vascularization, edema and pigmentation for up to 120 weeks. Clinically significant reductions in corneal pigmentation (P<0.0001), vascularization (P<0.0001) and edema scores (P<0.0001) were achieved during therapy. The greatest reduction was exhibited by corneal edema, closely followed by vascularization. Corneal pigmentation was less responsive and complete regression has not been achieved in any of the dogs. Severely affected corneas exhibited greater improvements in terms of pigmentation, whereas moderately affected corneas showed a slight decrease. Corneal edema and vascularization were adequately controlled, whereas corneal pigmentation exhibited a lower decrease in severity and was more difficult to manage on a long-term basis. This study suggests that subconjunctival injection of TA in addition to topical steroids could represent an effective option for long-term management of CSK in dogs unresponsive to topical steroids alone
Treatment outcomes of using paraosseous clamp and cerclage stabilisation technique in long bone fractures of cats: a retrospective study
(Ankara Üniversitesi, 2020-12-25) Şen, İlker; Sağlam, Mehmet; Veteriner Fakültesi
The stabilisation technique of paraosseous clamp and cerclage has become one of the most widely used current techniques because of the easy of applicability and low cost. It is a relatively new method providing rigid fixation in the treatment of fractures, especially those which do not have sufficient cortex resistance and have a wide medullar canal in the period when the long bones of the extremity have not completed development. The technique is based on binding 3 double cerclage wires to each other to form a paracortical belt. The aim of this study was to evaluate the clinical and radiological results of fractures in the extremity long bones of cats treated with the new stabilisation method of paraosseous clamp and cerclage. The study group was formed of 14 cats of various breeds and ages, of both genders, diagnosed with a fracture of the extremity long bone. Clinical and radiographical follow-up examinations were made at 10, 30, and 45 days postoperatively. Clinical healing was achieved in 13 cases on postoperative day 45, and those cases could use the affected extremity. A correlation was determined between the functional healing results and low complication rates, including unstable fractures, and it is supported that this technique could be use of this technique in the treatment of long bone fractures. In conclusion, the results of this study demonstrated that the paraosseous clamp and cerclage stabilisation technique can be used as an alternative method in the treatment of long bone fractures, especially in indicated cases.
Uterine papillary adenocarcinoma in a Pit-bull dog
(Ankara Üniversitesi, 2020-12-25) MONSEF, Yanad; KUTLU, Tuncer; KUTSAL, Osman; Veteriner Fakültesi
The uterus of an 18 month old Pit-bull removed during ovariohysterectomy formed the material of this study. Macroscopically, the uterus was whitish in color and elastic in consistency, the uterine horns were 15 cm long with a 1 cm of diameter. On cut section uterine mucosa showed variable sized cysts and was filled with a yellowish white mucus. Microscopical evaluation demonstrated a proliferation of the glandular component of the endometrium and a migration of these glands into the myometrium. Glands located in both the endometrium and the myometrium formed large cystic structures. The atypical glandular epithelium showed papillary projections into the cystic spaces. The tumor was diagnosed as uterine adenocarcinoma, an extremely rare canine tumor, especially in young dogs.

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