Browsing by Author "Sevin, Sedat"
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Item Acute fipronil intoxication in Squirrel Monkey (Saimiri sciureus)(Ankara Üniversitesi, 2021-03-31) Demir, Onur; Özdemir, Özgen; Koçak, Asiye; Arslan, Zeynel; Sevin, Sedat; Veteriner FakültesiA biocidal product in gel form containing 0.05% fipronil active substance was used for pest control in the habitat of thirteen Squirrel Monkey (Saimiri sciureus) in a zoo. Following the application, sudden death was observed in three primates. Routine bacteriological, histopathological and toxicological analyses on one cadaver and tissue samples from the cadaver did not produce any pathogenic agent, pesticide and any pathognomonic findings. In an attempt to investigate the presence of known fipronil intoxication biomarkers, fipronil and fipronil sulfone, we applied modified QuEChERS extraction processes on tissue samples and determined the fipronil and fipronil sulfone levels as 27.1-3.5 μg/kg in cutaneous tissue and 13.8-47.2 μg/kg in brain tissue, respectively, by using UHPLC Q-Orbitrap Mass Spectrometer. In the light of the anamnesis, necropsy, microbiology and histopathology results, and after evaluating the presence of fipronil-fipronil sulfone in tissues, we considered that the deaths could have resulted from acute fipronil toxicity due to species-specific active substance susceptibility.Item Effects of different chilling procedures on honey bees (Apis mellifera) for anesthesia(Ankara Üniversitesi, 2020-06-03) Tutun, Hidayet; Sevin, Sedat; Çetintav, Bekir; Veteriner FakültesiImmobilization is required for the examination and manipulation of honey bees (Apis mellifera), and many techniques have been developed to render honey bees immobile until this date. Among them, two methods, which are carbon dioxide (CO2) and low-temperature narcosis, have been commonly used to induce anesthesia on the bees. Although CO2-induced anesthesia changes the behavior and physiology of honey bees, the anesthesia induced by cold is considered commonly as non-damaging for the bees. There are a very limited number of studies on the effects of different chilling protocols in honey bees. Therefore, the purpose of this study was to investigate the effect of different chilling procedures on the survival rate and depth of anesthesia in honey bees. A total of 4 groups were formed, one of which was the control group. Three temperatures (+4°C, -20°C and -80°C) were applied to three different experimental groups. Furthermore, four different exposure times were implemented on each experimental group (n=20 worker bees in each trial). The depth of anesthesia was assessed and scored based on the movements and anesthesia recovery time in honeybees. There was a statistically significant relationship between duration and survival/death rate in the applications at +4°C, -20°C and -80°C (P<0.001). The method that was applied for 5 minutes at -20°C was the most ideal chilling method (P<0.03) and showed the lowest mortality in addition to the high depth of anesthesia. This study may assist in selecting the safest and deepest anesthesia method required in any study on honey bees.Item Investigation of the effects of Pine and Chestnut Honey on wound healing(Ankara Üniversitesi Veteriner Fakültesi, 2022) Sevin, Sedat; Veteriner; Veteriner FakültesiThis study aimed to investigate the effect of creams containing pine and chestnut honey on wound healing in rats. The animals were divided into the following four groups: a control group (A), a group treated with only basic cream ingredients (B), a group treated with pine honey cream (C), and a group treated with chestnut honey cream (D). Full-thickness skin wounds were created on the back of each rat (14 per group) with a 10-mm diameter punch instrument. In each group, skin samples were taken from seven rats on day 7 and from the remaining seven rats on day 14. The effects of the creams on wound healing were assessed by histopathological examination and hydroxyproline assays. The histopathological examination showed that chronic inflammation significantly decreased in group D compared to the control group (P<0.05). Furthermore, the levels of granulation tissue and granulation tissue/fibroblast maturation in skin samples taken on day 14 were also significantly lower in group D compared to the control group (P<0.05). It was observed that the neovascularization values of skin samples taken on day 7 were lower in group D than in the control group (P<0.05). Also, the difference in hydroxyproline levels between groups B and D was statistically significant (P<0.05). The results showed that the cream containing chestnut honey had a positive effect on wound healing.Item Ülkemize özgü çam balı ve kestane balı'nın krem tarzında farmasötik şeklinin geliştirilmesi ve yara iyileşmesi üzerine etkisinin araştırılması(Sağlık Bilimleri Enstitüsü, 2018) Sevin, Sedat; Yarsan, Ender; Veteriner HekimliğiApiterapi, arı ürünlerinin, bazı hastalıkların tedavisinde tamamlayıcı olarak kullanılmasıdır. Bu doktora çalışmasında, ülkemize özgü çam balı ve kestane balı içeren krem formülasyonlarınin geliştirilmesi ve bu formulasyonların açık yaralar üzerine iyileştirici etkilerinin belirlenmesi amaçlanmaktadır. Çalışmada, herhangi bir uygulama yapılmayan kontrol grubu (A), sadece krem taşıt maddesi uygulanan grup (B), Çam Balı kremi uygulanan grup (C) ve Kestane balı kremi uygulanan grup (D) olmak üzere 4 farklı denek grubu ile çalışılmıştır. İlk aşamada, her grupta (n=14) yer alan sıçanların sırt kısmına 10 mm çapında punch aparatı ile tam kat yara oluşturulmuştur. Oluşturulan yaraların yüzey alanını kapatacak şekilde hergün krem uygulaması yapılmıştır. Farklı gruplarda yer alan sıçanların yarısının deri örnekleri yara oluşumunu takip eden ilk 7. günde alınırken geri kalan yarısının deri örnekleri 14. günde alınarak çalışmanın sıçan uygulaması kısmı tamamlanmıştır. Alınan bu deri örneklerine; histopatoloji, hidroksiprolin ve yara iyileşme alanının belirlenmesi analizleri uygulanmıştır. Histopatolojik değerlendirme sonucuna göre, kronik yangının, kestane balı kremi uygulanan grupta kontrol grubuna göre önemli derecede azaldığı tespit edilmiştir (p<0,05). Buna ek olarak, granülasyon doku ve granülasyon dokusu/fibroblast olgunlaşması'nın da kestane balı kremi uygulanan ve 14. gün alınan deri örneklerinde, kontrol grubuna göre önemli derecede azaldığı belirlenmiştir (p<0,05). Kestane balı kremi uygulanan grubun 7.günde alınan deri örnekleri neovaskülarizasyon değerlerinin, kontrol grubu neovaskülarizasyon değerlerine göre daha düşük değerlerde olduğu gözlenmiştir (p<0,05). Ayrıca, hidroksiprolin düzeyleri çalışma grupları yönüyle değerlendirildiğinde B ve D grupları arasındaki farklılık istatistiksel açıdan önemli bulunmuştur (p<0.05). Sonuçlar bütün halinde ele alındığında, kestane balı içeren kremin yara iyileşmesi üzerine katkısının olumlu yönde olduğu belirtilmiştir. Bu çalışmadan elde edilen sonuçlar temel alınarak, ileriye dönük olarak farklı bal çeşitleri, farklı hayvan modelleriyle, doz çeşitliliği sağlayarak yeni bilimsel çalışmalar planlanabilir. Anahtar kelime: Apiterapi, çam balı, kestane balı, sıçan, yara iyileşmesiItem What has been done in the fight against Varroa destructor: from the past to the present(Ankara Üniversitesi Veteriner Fakültesi, 2022) Sevin, Sedat; Veteriner; Veteriner FakültesiBees are the major pollinators in natural ecosystems and in the agricultural production of several crops used for human consumption. However, they are exposed to multiple stressors that are causing a serious decline in their population. We highlight a major one among them, the Varroa destructor mite (Varroa) that causes severe impacts on the health of honey bee colonies, transmitting a variety of viruses that can affect the survival ability of individual bees and entire colonies. Diagnosis and mite control methods have been intensively studied in recent decades, with many studies in different areas of knowledge having been conducted. This overview summarizes these studies with a focus on colony defense systems, biological characteristics of the parasite Varroa, diagnostic methods used to establish the infestation level of colonies, and currently used control methods.