Cilt:30 Sayı:02 (2021)

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https://dspace.ankara.edu.tr/handle/20.500.12575/86634

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Browsing Cilt:30 Sayı:02 (2021) by Author "Biyoloji"

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    A NEW RARE MACROPHYTE RECORD FROM SW ASIA AND ECOLOGICAL CONDITIONS AT ITS HABITAT
    (Ankara Üniversitesi Fen Fakültesi, 2021) Yaprak, Ahmet Emre; Biyoloji; Fen Bilimleri Enstitüsü
    Aldrovanda vesiculosa L. is a cosmopolitan but very rare carnivorous aquatic angiosperm species from the family Droseraceae. The species was widespread and more common few decades ago but unfortunately it is being extinct in so many former distribution areas due to aquatic pollution and eutrophication. During the field trip to Karakuyu Lake we recognized a remarkable free-floating plant, after detailed investigation we identified the specimens as Aldrovanda vesiculosa which is a new record from Turkey and also SW Asia. In this publication, we provided detailed description of the species based on Turkish specimens, its habitat description, water physiochemical attributes, co-occurring macrophyte species list and botanical illustration of the species.
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    ACCUMULATION OF CR6+, PB2+ AND CD2+ AND ULTRAVIOLET RADIATION ALTER METHYLATION AND GENOMIC DNA STATUS IN RAMALINA FARINACEAE
    (Ankara Üniversitesi Fen Fakültesi, 2021) Aras, Emine Sümer; Biyoloji; Fen Fakültesi
    In this study was aimed to determine the genotoxic effect of Ramalina farinacea lichen species against stress sources at the molecular level. After applying three different heavy metals (Pb2+, Cd2+, and Cr6+) to the R. farinacea, the extent to which the lichen sample absorbed these metals was determined by Flame Atomic Absorption Spectroscopy. RAPD and MSAP-AFLP assays were also used to determine the status of DNA damage. The heavy metal analysis showed that R. farinacea had high levels of Pb2+, Cd2+, and Cr6+ content. According to the results obtained from molecular analyses, band changes were observed against seven primers heavy metal stresses and three primers against UV stress. An increase in Genomic Template Stability (GTS) was determined during the time in R. farinacea treated with all heavy metal concentrations. The effect of UV radiations in R. farinacea revealed the highest polymorphism and the lowest GTS rate depending on the dose. Among all methylation combinations, Type II was found to show altered in R. farinacea in response to Pb2+, Cd2+, and Cr6+ contents and UV radiations. R. farinacea can be used at the molecular level as a biomarker of suitable genotoxic effect. This is the first study to reveal DNA damage against stress sources using a sample of R. farinacea lichen species.
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    EFFECTS OF LIGHT AND SALINITY ON THE GERMINATION OF CLOSELY RELATED THREE SALSOLA TAXA
    (Ankara Üniversitesi Fen Fakültesi, 2022) Tuğ, Gül Nihal; Biyoloji; Fen Fakültesi
    Three closely related Salsola taxa (Salsola boissieri Botsch. subsp. serpentinicola (Freitag & Özhatay) Freitag & Uotila, Salsola boissieri Botsch. subsp. boissieri, Salsola turcica Yıldırımlı (halophytic ecotype), Salsola turcica Yıldırımlı (gypsicole ecotype)) from different edaphic conditions were studied according to changing light and salinity conditions. Seeds of target taxa were collected in 2017 and their weights were determined. The perianth segments were removed before the experimental trials and all the trials were conducted at 9°C/22°C which is the mean night and day temperatures of germination season. For the determination of the influence of light, one set of seeds for distilled water trial were kept at complete darkness. Different NaCl concentrations (distilled water, 100, 200, 300 mM NaCl) were used to evaluate the effects of salinity on germination. Viability of the seeds were determined by Triphenyl Tetrazolium Chloride (TTC) test which was applied to the seeds that did not germinate during the trials. As a result, it was found out that light stimulates germination of the taxa and Salsola seeds showed better germination ratio at light. The most tolerant taxa against salinity are the halophytic and gypsicole ecotypes of S. turcica, and the most susceptible one is S. boissieri subsp. serpentinicola. Both of the species show reduced germination ratios with increasing salinity. Salinity tolerance of S. boissieri subsp. serpentinicola and S. boissieri subsp. boissieri are very low, according to the Decreasing Germination Percentage (DGP) values. Although they show different germination response against increasing salinity, there is not any statistically meaningful difference between these three taxa according to germination percentages at different salinities, germination rates, last germination ratios and seed viabilities (F=1.818 p>0.05) (One Way ANOVA, SPSS 25).