Cilt:69 Sayı:02 (2022)

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https://dspace.ankara.edu.tr/handle/20.500.12575/85300

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Browsing Cilt:69 Sayı:02 (2022) by Author "Other"

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    Effects of replacing grain feed with rumen-protected fat on feedlot performance, ruminal parameters and blood metabolites in growing Merino lambs’ diets during the hot season
    (Ankara Üniversitesi Veteriner Fakültesi, 2022) Gümüş, Hıdır; Other; Other
    In this study, the effects of rumen-protected fat (RPF) were evaluated as an energy source for partial replacement of grain feed in the diets of Merino male lambs during the hot season. Fourteen six months old Merino male lambs weighing average of 29.59 kg were randomly allocated into two groups: control diet (CON) and diets supplemented with 30 g/kg RPF. Live weight (LW), dry matter intake (DMI) and average daily gain (ADG) were recorded, and the feed conversion ratio (FCR) was then calculated. The ruminal pH, the ruminal ammonia nitrogen concentrations (NH3-N) value, the molar proportion of volatile fatty acid (VFA), blood serum as well as hematological parameters were determined and protozoa numbers were counted. While the live weight and daily gain of the lambs were not influenced by dietary treatments, DMI was increased with RPF supplementation. The ruminal pH, VFA and ruminal NH3-N concentrations were unchanged, but the protozoa numbers decreased significantly by RPF supplementation (P<0.05). RPF supplementation significantly affected some serum and hematological parameters in comparison to the control group. In conclusion, RPF may be added to concentrate mixture lamb feed at the level of 30 g/kg to enhance the diet energy density without negatively changing the animal’s performance.
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    Ev Yapımı Elma ve Üzüm Sirkelerinin Kimyasal, Antibakteriyel ve Antiradikal Özelliklerinin Araştırılması
    (Ankara Üniversitesi Veteriner Fakültesi, 2022) Kahraman, Hatice Ahu; Other; Other
    Bu çalışma, ev yapımı elma ve üzüm sirkelerinin başlıca uçucu bileşikleri, organik asit, fenolik ve mineral içerikleri ile antiradikal ve antimikrobiyal özelliklerini araştırmayı amaçlamıştır. Üzüm sirkesinin elma sirkesine göre daha yüksek toplam asitlik, organik asit içeriği, toplam fenolik içerik (TPC) ve antiradikal aktiviteye sahip olduğu, aynı zamanda daha düşük minimum inhibitör konsantrasyon (MIC) değerleri gösterdiği tespit edildi. Üzüm sirkesinde asetik ve tartarik asitler en çok bulunan organik asitti. Elma sirkesinde ise en çok bulunan organik asitler asetik ve süksinik asitlerdi. Her iki sirke çeşidinde de en fazla bulunan fenolik bileşik gallik asitti. Üzüm ve elma sirkelerinin sırasıyla 18 ve 9 adet uçucu bileşik içerdiği belirlendi. Üzüm sirkesinde asetik asit ve asetoin en çok bulunan uçucu bileşikler iken; asetik asit, etil asetat, 2,4,5-trimetil-1,3-dioksolan elma sirkesinde en fazla bulunan uçucu bileşiklerdi. K, Ca ve Na, her iki sirkede en yaygın bulunan mineraller iken; üzüm sirkesinde elma sirkesine kıyasla daha fazla oranda bulundukları tespit edildi. Üzüm sirkesinde test edilen bakterilere karşı MIC değerlerinin % 6.25 olduğu, Minimum bakterisidal konsantrasyon (MBC) değerlerinin % 6.25 ile % 12.50 arasında değiştiği görüldü. Elma sirkesinin test edilen bakterilere karşı MIC değerleri % 12.50 olduğu, MBC değerlerinin ise tüm test bakterileri için % 12.50 ile % 25.00 arasında değiştiği görüldü. Sonuç olarak sirkelerin sahip olduğu antiradikal ve antibakteriyel aktivitelerinin, TPC, organik asit içerikleri ile ilişkili olduğunu düşünülmektedir.
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    Explant culture and multilineage differentiation of amniotic membrane derived stem cells
    (Ankara Üniversitesi Veteriner Fakültesi, 2022) Akkaya, Özlem Özden; Other; Other
    Amniotic membrane derived stem cells (AMSCs) are reported to have a comparatively higher potency than multipotent stem cells. These cells are shown to have low immunogenicity and no teratogenicity. Among various conventional methods of isolation using enzymes, explant culture method is believed to be an easy and cost-effective way to harvest stem cells. The purpose of this study was to isolate AMSCs from amniotic membrane of rats and to characterize them for multilineage differentiation, including generation of neurospheres to use them later in in-vivo experiments. Amniotic membranes were collected from Wistar rats on 17th day of pregnancy. After processing of the tissues, AMSCs were isolated by the explant culture method and continued to grow until 10th passage. The doubling time was estimated and the cells were analyzed for growth curve parameters at passages 5 and 9. The osteogenic and adipogenic differentiation studies were carried out from the same cells after 3rd passage. Neurospheres generation from AMSCs was performed using neurogenic induction media. The cells were further assessed for their mesenchymal, haemopoietic, and neurogenic marker expressions by immunofluorescence staining and PCR analysis The study suggests that AMSCs isolated through explant culture are reliable stem cells which could generate neurospheres under proper induction conditions and could be a potential candidate to be used on in-vivo neural degeneration models.
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    Fecal microbiota transplantation capsule therapy via oral route for combatting atopic dermatitis in dogs
    (Ankara Üniversitesi Veteriner Fakültesi, 2022) Ural, Kerem; Other; Other
    Given the role of the interaction between gut microbiome with dermatological diseases, namely “gut-skin axis”, the present author proved that gut restoration should alleviate canine atopic dermatitis (CAD), which was the purpose of the study. A 4-week, open-label, non-controlled case series involved 8 -owned dogs with CAD which had received no previous treatment. Evaluations included Canine Atopic Dermatitis Extent and Severity Index version 4 (CADESI-04 scores), Visual Analog Scale (VAS) pruritus scores and Polycheck in vitro allergen specific tests. Faecal samples were analysed by dual indexing one-step PCR and 16S rRNA targeted metagenomics for detecting gut microbiota alterations before and after fecal microbiota transplantation (FMT) capsule treatment twice daily for 4 weeks. All cases were presenting pruritus and all of those dogs showed elevated IgE levels. CADESI scores decreased on days 28 (4-21) compared to day 0 initial values (50-128). Similarly, decreased VAS scores were detected on days 28 (0-2) in contrast to prior values (6-10). Regarding epidermal barrier functioning epidermal hydration (55-100 vs. 4-24) and pH (6.-7.8 vs. 4.2-5.7) values were elevated after FMT treatment in contrast to prior ranges, respectively. Alpha diversity revaled both richness and diversity of gut microbiota were improved for all cases on day 28. Furthermore at the end of trial Firmicutes: Bacteroidetes ratio was 8, the benchmark detected for healthy dogs. The present study supports a potential benefit of FMT capsule treatment against CAD. This safe and tolerant treatment modality directed against CAD shifted the gut microbiome composition towards a healthy state for all 8 dogs enrolled.
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    Simental Irkı Bir Buzağıda Şilognatopalatoşizi Olgusu: Nadir Bir Olgu
    (Ankara Üniversitesi Veteriner Fakültesi, 2022) Polat, Eren; Other; Other
    Bu çalışmanın konusunu, üst dudak ve damağında yarık şikâyeti ile Fırat Üniversitesi Veteriner Fakültesi Hayvan Hastanesi Cerrahi Kliniği’ne getirilen 7 günlük, dişi simental ırkı bir buzağı (7 günlük yaşta, dişi) oluşturdu. Yapılan klinik muayenede, buzağının solunum güçlüğünün olduğu ve burun deliklerinin normal olarak şekillenmediği tespit edildi. Buzağıya cheilognathopalatoschisis teşhisi konuldu. Nadiren karşılaşılan olgunun tedavisi için buzağıya palatoplasti ve rekonstrüktif cerrahi uygulandı. Yarık olan sert damağın her iki ucu serbestleştirilerek karşılıklı dikildi. Burun deliklerinin ise medial kanatları yara oluşturularak dikilip normal pozisyonuna getirildi. Hasta sahibi ile yapılan görüşmede buzağının beslenmesi sırasında yutma zorluğu bulunduğu öğrenildi. Postoperatif 8. gün buzağının olası yutma refleksinin oluşmamasından dolayı gelişen aspirasyon pnoumonisi sebebiyle öldüğü belirlendi.
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    The effects of adding waste sesame seeds to diets on performance, carcass characteristics, and meat fatty acid composition of Karayaka lambs
    (Ankara Üniversitesi Veteriner Fakültesi, 2022) Kaya, İsmail; Other; Other
    This study aims to determine the effects of the inclusion of waste sesame seed (WSS) to concentrate feed on performance, carcass characteristics, and meat fatty acid profile of Karayaka lambs. A total of twenty-three lambs were randomly allocated to a control group (11 lambs, with an average live weight of 23.39±0.58 kg) and an experimental group (12 lambs, with an average live weight 23.55±0.41 kg). Also, each of the group was divided into two subgroups according to their gender. The lambs were fed with isonitrogenous (17% CP) and isocaloric (2650 kcal/kg ME) concentrate feeds containing either 0% WSS (control) or 10% WSS (experimental) for 60 days. Final live weight, average daily weight gain (ADG), feed conversion ratio (FCR), and carcass traits were not affected by diet, although they were higher in males than females (P<0.01). In addition, males presented a higher percentage of C17:1, C18:2 n6, C20:3 n6 (P<0.01), and polyunsaturated fatty acid (P<0.05) than females. In conclusion, the addition of WSS to the lamb diet did not increase the concentration of unsaturated fatty acids in meat. However, WSS can be included up to 10% in concentrate feed of lambs during fattening period without negative effects on performance and carcass yield.
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    The evaluation of the therapeutic potential of hesperetin on diethylnitrosamine and phenobarbital induced liver injury in rats
    (Ankara Üniversitesi Veteriner Fakültesi, 2022) Kısaçam, Mehmet Ali; Other; Other
    Nitrite and amine reactions can occur rapidly and produce nitrosamines, in-vivo. Diethylnitrosamine (DEN) and phenobarbital (PB) are readily inducing liver injury and hesperetin (HES), as a flavonoid found in citrus fruits, have the potential to compensate for their harmful effects. In this study, the therapeutic effects of HES were evaluated in DEN and PB mediated liver defect. Adult male Sprague-Dawley rats were split into 5 groups (n=10): Control, DEN, DEN+PB, HES, and DEN+PB+HES. 150 mg/kg DEN was applied intraperitoneally to DEN groups. Fifteen days after the DEN application 500ppm of PB was given in drinking water. HES were administered at 50 mg/kg dose orally for 8 weeks. Blood and liver malondialdehyde (MDA), glutathione (GSH) levels, and catalase (CAT), superoxide dismutase (SOD) activities were measured spectrophotometrically. Moreover, histologic examination of liver sections and apoptosis were determined with hematoxylin-eosin and TUNEL methods, respectively. DEN-PB application was found to increase blood and liver MDA levels and liver CAT activity, oppositely, decreased blood and liver SOD activity, GSH levels, and blood CAT activity. HES was found to have a positive impact on oxidative stress parameters by decreasing liver and blood MDA activity, increasing blood CAT and SOD activity together with liver GSH levels and SOD activity. Whereas DEN and PB application increased all histopathological findings and TUNEL positive cells, HES administration decreased these findings which might be important for the protection of liver cell structure from cell damage. These results suggest that HES administration could be an alternative therapeutic approach to liver damage.