Cilt:30 Sayı:01 (2021)
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Item Bıochemıcal and pathologıcal effects on the male rat hepatıc tıssue after exposure to 900mhz electromagnetıc fıeld durıng adolescent perıod(Ankara Üniversitesi Fen Fakültesi, 2021) Keleş, Ayşe İkinci; Other; OtherThe aim of this study was to employ biochemical analysis and histopathological techniques to investigate the effects of the application of a 900-megahertz electromagnetic field during adolescence on the rat liver. 24 male Sprague Dawley rats aged 21 days were randomly divided into electromagnetic field, sham and control groups. No procedure was performed on the control group rats. Electromagnetic field group rats were exposed to a 900-megahertz electromagnetic field in an electromagnetic field application cage (1 hours/day, every day for 25 days). The sham group rats were placed in the same electromagnetic field cage without exposure to electromagnetic field. At the end of the experiments, the livers were removed. The livers were used for histopathological evaluation (light, electron and immunofluorescence microscopy) and biochemical analyses. Biochemical analyses revealed increased lipid peroxidation and glutathione values while catalase and superoxide dismutase values were decreased in electromagnetic field group. Electron microscopy evaluations identified necrotic hepatocytes with numerous cytoplasmic and mitochondrial vacuoles in electromagnetic field group sections. We also observed an enlarged endoplasmic reticulum and loss of mitochondrial matrix, in addition to the presence of vacuoles. Also, loss of inner and outer mitochondrial membrane integrity was observed. Light microscopy evaluations revealed sinusoidal capillary dilatation and degeneration in hepatocytes with cytoplasmic swelling, vacuolar degeneration and pyknotic nuclei in electromagnetic field group sections. Deterioration in the integrity of the collagen fibers was also present around the vena centralis. Additionally, electromagnetic field had no effect on body weight. Therefore, we conclude that continuous 900-megahertz electromagnetic field treatment may cause changes in oxidative stress biomarkers and the morphology of the adolescent rat liver.Item Characterızatıon of ıts1 secondary structure ın ten specıes of coluteocarpeae (brassıcaceae) and ıts taxonomıcal utılıty(Ankara Üniversitesi Fen Fakültesi, 2021) Özgişi, Kurtuluş; Other; OtherUtility of the internal transcribed spacers (ITSs) of ribosomal RNA sequences to infer phylogenetic relationships among organisms have been proven. Although ITS1 and ITS2 are highly variable in sequence, they have conserved structures that have a key function in the processing of rRNA gene transcripts. Determining of such a conserved motif can help to identify relationships between organisms. Since ITS2 has much more conserved secondary structure, structural properties of ITS1 are generally neglected by researchers. In this study, ITS1 secondary structures of ten representative species, which were once assigned under different genera, of tribe Coluteocarpeae were determined. Also taxonomical utility of ITS1 secondary structure was also tested. Analyses indicate that there are four different types (4-, 6-, 7- and 8 hairpin) of secondary structures. On the other hand, each sequences have a conserved region that is common among land plants. Since previous studies reveals other species, that belong different tribes or lineages of Brassicaceae show similar ITS1 secondary structure, it is not a useful delimitation tool for investigated species in terms of taxonomy.Item New localıty records for eumeces schneiderii (daudin, 1802) and trapelus ruderatus (olivier, 1804) wıth the morphologıcal data from eastern anatolıa, Turkey(Ankara Üniversitesi Fen Fakültesi, 2021) Şahin, Mehmet Kürşat; Other; OtherIn this study, we report new locality records for Eumeces schneiderii and Trapelus ruderatus from Malatya, Eastern Anatolia. As the new locality is about 60 km north of the known distribution area for E. schneiderii from Malatya and T. ruderatus is the first record for this province. A summary of metric and meristic characteristics was with color-pattern features was given for these specimens. Moreover, the explored locality’s importance for the species biogeography via Anatolian diagonal was emphasized.Item Taxonomıc contrıbutıons to the genus dıanthus (caryophyllaceae) from Turkey(Ankara Üniversitesi Fen Fakültesi, 2021) Hamzaoğlu, Ergin; Other; OtherAccording to the related literature and some taxonomic websites, it was proven that Dianthus liboschitzianus and D. erinaceus (var. erinaceus) are the valid names, which are accepted as a synonym. It was determined that Dianthus erinaceus var. alpinus not D. erinaceus (var. erinaceus) is a taxon, which is a synonym under Dianthus webbianus. Furthermore, the lectotypes were determined for Dianthus erinaceus and D. masmenaeus var. glabrescens, which was typed by being based on more than one specimen.