Analysis of Prion Protein Coding Gene Polymorphisms in Palestinian Native Sheep Breeds

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dc.contributor.authorÜn, Cemal
dc.contributor.authorAlak, Sedef Erkunt
dc.contributor.authorAlsayed, Osama
dc.contributor.departmentVeteriner Fakültesitr_TR
dc.date.accessioned2021-10-06T13:45:41Z
dc.date.available2021-10-06T13:45:41Z
dc.date.issued2019-06-14
dc.description.abstractPrion protein coding gene (PRNP) is the genetic locus correlated with the greatest impact on classical scrapie susceptibility in sheep. At codons 136, 154, and 171 of PRNP alanine/arginine/glutamine (ARQ) and valine/arginine/glutamine (VRQ) haplotypes, in turn, are related to susceptibility to classical scrapie while alanine/arginine/arginine ARR haplotype is correlated with resistance. The aim of the present study was to genotype the Palestinian native sheep breeds for detection of genetic resistance. A total of 38 healthy sheep from Awassi and Assaf breeds were randomly sampled. Genomic DNA was isolated from blood samples. After PCR amplification and DNA sequencing, ARQ, ARR, ARH, AHQ, ARL and VRQ alleles and ARR/ARQ, ARQ/ARQ, ARQ/ARL, ARH/ARQ, ARH/ARL, AHQ/ARQ and ARQ/VRQ genotypes were detected in PRNP gene. ARQ allele was found as a predominant allele in this study with the frequency of 0.76 for Awassi and Assaf breeds while the uncommon allele ARL was identified at low frequencies in both breeds. In addition, two different polymorphisms were recognized (V12I and L23H) at different codons of PRNP. Results have indicated that most of the genotypes belong to risk group 3. The careful dissemination of ARR/ARR sheep is suggested to increase resistant allele frequencies in Assaf and Awassi breeds.tr_TR
dc.identifier.endpage266tr_TR
dc.identifier.issn/e-issn1308-2817
dc.identifier.issue3tr_TR
dc.identifier.startpage261tr_TR
dc.identifier.urihttps://doi.org/10.33988/auvfd.437314tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/75270
dc.identifier.volume66tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.33988/auvfd.437314tr_TR
dc.relation.journalAnkara Üniversitesi Veteriner Fakültesi Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectAssaftr_TR
dc.subjectAwassitr_TR
dc.subjectPalestiniantr_TR
dc.titleAnalysis of Prion Protein Coding Gene Polymorphisms in Palestinian Native Sheep Breedstr_TR
dc.typeArticletr_TR

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