Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir

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dc.contributor.authorAtalay, Merve
dc.contributor.authorŞahingil, Didem
dc.contributor.departmentOthertr_TR
dc.contributor.facultyOthertr_TR
dc.date.accessioned2022-09-13T07:11:16Z
dc.date.available2022-09-13T07:11:16Z
dc.date.issued2022
dc.description.abstractIn the present study, bioactive properties such as ACE-I activity and antimicrobial activity of kefir using different Lactobacillus (Lactobacillus delbrueckii ssp. bulgaricus ATCC 11842, Lactobacillus helveticus ATCC 15009 and Lactobacillus plantarum ATCC 14917) on some pathogen and Gram-positive bacteria during 28 day-storage periods was investigated and proteolysis (RP-HPLC peptide profiles, RP-HPLC amino acid profiles) were studied. The antimicrobial activity was investigated in kefir extract and separated peptide fractions (<3 kDa, named F2) which were characterized by LC-MS/MS and precursor and product ions were determined. Antimicrobial activity has been observed against pathogenic bacteria such as Escherichia coli (E. coli) in all samples. But the results revealed that the F2 fraction separated from kefir manufactured using Lactobacillus had a stronger antibacterial effect than control samples. It was determined that the F2 fraction has antimicrobial activity against S. aureus, S. warneri 95052 and S. hominis. The ACE-I activity of samples A, B, C and K were 76.47%, 84.95%, 87.33% and 85.57%, respectively. In the kefir using Lactobacillus has increase of ACE-I activity and was significant (P<0.01). In this study, it was concluded that the using of adjtr_TR
dc.description.indexWostr_TR
dc.description.indexScopustr_TR
dc.identifier.endpage384tr_TR
dc.identifier.issn/e-issn2148-9297
dc.identifier.issue3tr_TR
dc.identifier.startpage372tr_TR
dc.identifier.urihttps://doi.org/10.15832/ankutbd.880744tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/83938
dc.identifier.volume28tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.15832/ankutbd.880744tr_TR
dc.relation.journalAnkara Üniversitesi Ziraat Fakültesi Tarım Bilimleri Dergisitr_TR
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Başka Kurum Yazarıtr_TR
dc.subjectBioactive peptides,tr_TR
dc.subjectIn-Vitro antimicrobial peptidestr_TR
dc.titleCharacterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefirtr_TR
dc.typeArticletr_TR

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Cilt:28 Sayı:03 (2022)