A direct tissue-grafting approach to increasing endogenous brown fat

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dc.contributor.authorKarakecili, Ayse
dc.contributor.departmentMühendislik Fakültesitr_TR
dc.date.accessioned2020-12-30T12:56:34Z
dc.date.available2020-12-30T12:56:34Z
dc.date.issued2018
dc.description.abstractThere is widespread evidence that increasing functional mass of brown adipose tissue (BAT) via browning of white adipose tissue (WAT) could potentially counter obesity and diabetes. However, most current approaches focus on administration of pharmacological compounds which expose patients to highly undesirable side effects. Here, we describe a simple and direct tissue-grafting approach to increase BAT mass through ex vivo browning of subcutaneous WAT, followed by re-implantation into the host; this cell-therapy approach could potentially act synergistically with existing pharmacological approaches. With this process, entitled "exBAT", we identified conditions, in both mouse and human tissue, that convert whole fragments of WAT to BAT via a single step and without unwanted off-target pharmacological effects. We show that ex vivo, exBAT exhibited UCP1 immunostaining, lipid droplet formation, and mitochondrial metabolic activity consistent with native BAT. In mice, exBAT exhibited a highly durable phenotype for at least 8 weeks. Overall, these results enable a simple and scalable tissue-grafting strategy, rather than pharmacological approaches, for increasing endogenous BAT and studying its effect on host weight and metabolismtr_TR
dc.description.indexPubmed
dc.identifier.endpage12tr_TR
dc.identifier.issue7957tr_TR
dc.identifier.startpage1tr_TR
dc.identifier.urihttps://doi.org/10.1038/s41598-018-25866-ytr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/72615
dc.identifier.volume8tr_TR
dc.language.isoentr_TR
dc.relation.isversionof10.1038/s41598-018-25866-ytr_TR
dc.relation.journalSci Rep .tr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjecttissue-grafting approachtr_TR
dc.titleA direct tissue-grafting approach to increasing endogenous brown fattr_TR
dc.typeArticletr_TR

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