Effects of Donor x Inducer Interaction on the Success of Haploid Induction and Comparison of Haploid Seed Identification Methods in the In vivo Maternal Haploid Technique in Maize

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dc.contributor.authorKahrıman, Fatih
dc.contributor.authorSongur, Umut
dc.contributor.authorDişbudak, Abdullah
dc.contributor.authorKızık, Sezgin
dc.contributor.authorVural, Berk
dc.contributor.departmentOthertr_TR
dc.contributor.facultyOthertr_TR
dc.date.accessioned2022-09-13T07:14:54Z
dc.date.available2022-09-13T07:14:54Z
dc.date.issued2022
dc.description.abstractThis study was conducted to investigate the effect of donor and inducer lines on the haploid induction rate in the in vivo maternal doubled haploid technique in maize and to compare the methods used in haploid seed separation. In the study, three donor materials were used for induction crossing with two inducer lines. Seedling immersion and stem injection techniques were used as chromosome doubling methods. Eye separation, spectral measurements and image processing were used to identify seed classes. Data were modeled using support vector machine method and created models were evaluated over the complexity matrix. The results of the research revealed that the haploid induction rate varies depending on the donor genotypes and inducers, and the genotype responses against the applied chromosome doubling method were also different. Differences were observed in the success of the three methods compared for haploid and diploid seed separation. The successful classification rates were 87%, 83%, and 79% in visual, spectral and image processing models, respectively. Results showed that both spectral technique and image processing technique can be used to distinguish haploid/diploid seeds in in vivo maternal haploid technique.tr_TR
dc.description.indexWostr_TR
dc.description.indexScopustr_TR
dc.identifier.endpage395tr_TR
dc.identifier.issn/e-issn2148-9297
dc.identifier.issue3tr_TR
dc.identifier.startpage385tr_TR
dc.identifier.urihttps://doi.org/10.15832/ankutbd.824114tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/83941
dc.identifier.volume28tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.15832/ankutbd.824114tr_TR
dc.relation.journalAnkara Üniversitesi Ziraat Fakültesi Tarım Bilimleri Dergisitr_TR
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Başka Kurum Yazarıtr_TR
dc.subjectSeed classification,tr_TR
dc.subjectSpectral analysis,tr_TR
dc.subjectPloidy, Chromosome doublingtr_TR
dc.titleEffects of Donor x Inducer Interaction on the Success of Haploid Induction and Comparison of Haploid Seed Identification Methods in the In vivo Maternal Haploid Technique in Maizetr_TR
dc.typeArticletr_TR

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Cilt:28 Sayı:03 (2022)