Employing Barcode High-Resolution Melting Technique for Authentication of Apricot Cultivars

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dc.contributor.authorHürkan, Kaan
dc.contributor.departmentOthertr_TR
dc.contributor.facultyOthertr_TR
dc.date.accessioned2022-09-13T10:53:57Z
dc.date.available2022-09-13T10:53:57Z
dc.date.issued2022
dc.description.abstractFast, accurate and affordable identification of food products is important to ensure authenticity and safety. There are various apricot (Prunus armeniaca L.) cultivars are being produced in Turkey. Each cultivar differs in quality and purpose of use. In this paper, we aimed to develop an easy and reliable method, Barcode High-Resolution Melting (Bar-HRM), to distinguish apricot cultivars. We designed and tested novel Bar-HRM primer sets HRM-ITS1 and HRM-ITS2, targeting the most popular barcoding region ITS1 and ITS2, specific to apricot cultivars. According to the results, HRM analysis distinguished 31 cultivars of 35 for ITS1, and 35 for ITS2. We recommend using ITS2 barcode region, amplified with using HRM-ITS2 primer set, for Bar-HRM analysis of different apricot cultivars.tr_TR
dc.description.indexWostr_TR
dc.description.indexScopustr_TR
dc.identifier.endpage258tr_TR
dc.identifier.issn/e-issn2148-9297
dc.identifier.issue02tr_TR
dc.identifier.startpage251tr_TR
dc.identifier.urihttps://doi.org/10.15832/ankutbd.870528tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/84003
dc.identifier.volume28tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.15832/ankutbd.870528tr_TR
dc.relation.journalAnkara Üniversitesi Ziraat Fakültesi Tarım Bilimleri Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Başka Kurum Yazarıtr_TR
dc.subjectMolecular Identificationtr_TR
dc.subjectInternal Transcribed Spacertr_TR
dc.subjectPrunus armeniacatr_TR
dc.titleEmploying Barcode High-Resolution Melting Technique for Authentication of Apricot Cultivarstr_TR
dc.typeArticletr_TR

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Cilt:28 Sayı:02 (2022)