Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock

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dc.contributor.authorÖngör, Hasan
dc.contributor.authorTimurkaan, Necati
dc.contributor.authorÇöven, Fethiye
dc.contributor.authorKarabulut, Burak
dc.contributor.authorEröksüz, Hatice
dc.contributor.authorÇetinkaya, Burhan
dc.contributor.departmentVeteriner Fakültesitr_TR
dc.date.accessioned2021-10-11T14:11:46Z
dc.date.available2021-10-11T14:11:46Z
dc.date.issued2021-03-31
dc.description.abstractThe aim of this study was to determine an infectious bronchitis (IB) infection, caused by an Israel variant 2 (IS/1494/06)-like IBV, in a layer chicken flock regularly vaccinated with vaccines containing IBV H120 and 4/91 strains. Mild respiratory symptoms, drop in egg production and soft-shelled eggs and eventually death were observed in a layer chicken flock. Clinical samples from four diseased chickens were examined for the detection and genotyping of IBV by virus isolation, a commercial real time reverse transcription polymerase chain reaction (rRT-PCR) and nucleotide sequencing. Both Israel variant 2 (IS-Var2) and 793/B serotypes were detected from samples by rRT-PCR, but sequencing results of a 345 bp part of S1 gene revealed that our IBV isolate, HFT-IBV, was IS/1494/06 (IS-Var2)-like with the 97.7% genetic similarity. These results suggested that immunity against vaccination with a combination of different genotypes (H120 and 4/91) could not be protective for IS-Var2 IBV field infection. In addition, identification of genotypes from the clinical samples, such as swabs and organ samples by commercial rRT-PCR assays failed to find correct IBV genotype responsible for the IB infection, whereas analysis of nucleotide sequencing of S1 gene of IBV as a gold standard test undoubtedly detected causative genotype of the infection. Also, the findings indicated that there is an urgent need for consider genotype- or protectotype-match vaccination strategies in the field to prevent vaccine- and IB-dependent economic losses of the poultry sector and logically protect chickens from IBV infection.tr_TR
dc.identifier.endpage172tr_TR
dc.identifier.issn/e-issn1308-2817
dc.identifier.issue2tr_TR
dc.identifier.startpage167tr_TR
dc.identifier.urihttps://doi.org/10.33988/auvfd.756970tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/75386
dc.identifier.volume68tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.33988/auvfd.756970tr_TR
dc.relation.journalAnkara Üniversitesi Veteriner Fakültesi Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectIsrael variant 2tr_TR
dc.subjectGenotypetr_TR
dc.subjectInfectious Bronchitis Virustr_TR
dc.titleDetection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flocktr_TR
dc.typeArticletr_TR

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