Molecular detection and phylogenetic analysis of Theileria equi and Babesia caballi in wild horses in Konya province of Turkey

dc.contributor.authorEkici, Özlem Derinbay
dc.contributor.authorCeylan, Onur
dc.contributor.authorSöznmez, Gonca
dc.contributor.authorDik, Bilal
dc.contributor.authorCeylan, Ceylan
dc.contributor.authorCeylan, Ceylan
dc.contributor.authorSemmassel, Asma
dc.contributor.departmentVeteriner Fakültesitr_TR
dc.date.accessioned2021-10-12T06:55:29Z
dc.date.available2021-10-12T06:55:29Z
dc.date.issued2021-06-30
dc.description.abstractThe aim of this study was to investigate equine piroplasms of wild horses (Equus ferus caballus) in Konya province of Turkey in November-December 2017. For this aim, blood samples were collected from 36 wild horses and examined for equine piroplasms by microscopy and multiplex PCR. Some of the PCR products from positive samples were also sequenced. Five (13.89%) out of the 36 horses were infected with either Theileria equi, Babesia caballi or both in the microscopical examination. Single infections with T. equi and B. caballi were detected in three (8.33%) and one horses (2.78%), respectively. Prevalence of T. equi, B. caballi and mix infections was determined as 50%, 38.8% and 38.8% by multiplex PCR, respectively. Multiplex PCR was found more sensitive than microscopical examination to detect the piroplasms of horses. The results of sequence analysis showed 99.25-100% and 98.23-99.59% nucleotide sequence identity to the previously reported T. equi and B. caballi 18S rRNA gene sequences, respectively. Consequently, the existence of equine piroplasmosis in wild horses was reported for the first time in Turkey, and high molecular prevalences of T. equi and B. caballi were reported with this study.tr_TR
dc.identifier.endpage281tr_TR
dc.identifier.issn/e-issn1308-2817
dc.identifier.issue3tr_TR
dc.identifier.startpage275tr_TR
dc.identifier.urihttps://doi.org/10.33988/auvfd.708329tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/75402
dc.identifier.volume68tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.33988/auvfd.708329tr_TR
dc.relation.journalAnkara Üniversitesi Veteriner Fakültesi Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectBabesia caballitr_TR
dc.subjectMultiplex PCRtr_TR
dc.subjectTheileria equitr_TR
dc.titleMolecular detection and phylogenetic analysis of Theileria equi and Babesia caballi in wild horses in Konya province of Turkeytr_TR
dc.typeArticletr_TR

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