Effect of Parental Genotypes and Their Reciprocal Crosses on Haploid Plant Production by Anther Culture and Confirmation of Double Haploids by Flow Cytometry in Bread Wheat
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Date
2022
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Journal ISSN
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Publisher
Ankara Üniversitesi
Abstract
Double haploids (DHs) production and utilization is an important aspect
of wheat breeding programs in worldwide, because, it provides many
advantages over the conventional breeding program. Therefore, in vitro
and in vivo responses of anther derived two bread wheat cultivars and
their F1 crosses were investigated. Results showed significant genetic
variation among the tested bread wheat genotypes. The pre-cold treated
anther derived embryonic calli and shoot induction (%) were obtained
from all genotypes that ranged 44 - 76% and 32 - 58% respectively. The
highest shoot induction from embryonic calli was achieved from F1
cross of Zubkov x Atay-85, whereas, lowest induction was obtained
from Atay-85 cultivar. The F1 cross (Zubkov x Atay-85) showed a
better response in anther culture in term of shoots per plant and root
induction than their respective parental genotypes.
The present results indicated positive and significant heterotic
effects of F1 crosses for calli and shoot induction that also showed less
albino plant regeneration after colchicine treatments. Total number of
114 anthers driven DHs lines from two genotypes and their F1 crosses
were regenerated, confirmed by flow-cytometry and evaluated under
field condition. Some DHs lines were found to be significantly superior
for agronomical traits including seed yield than the parental genotypes
as well as local bread wheat (control) cultivars.
Description
Keywords
Anther culture,, Bread wheat,, Colchicine,, Double haploid