Bovine Herpesvirus Type 4 (BoHV-4) Vector Delivering Nucleocapsid Protein of Crimean-Congo Hemorrhagic Fever Virus Induces Comparable Protective Immunity against Lethal Challenge in IFNα/β/γR-/- Mice Models
| dc.contributor.author | Farzani, Touraj Aligholipour | |
| dc.contributor.department | Veteriner Fakültesi | tr_TR |
| dc.date.accessioned | 2020-11-13T06:12:16Z | |
| dc.date.available | 2020-11-13T06:12:16Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Crimean-Congo hemorrhagic fever virus (CCHFV) is the causative agent of a tick-borne infection with a significant mortality rate of up to 40% in endemic areas, with evidence of geographical expansion. Due to a lack of effective therapeutics and control measures, the development of a protective CCHFV vaccine remains a crucial public health task. This paper describes, for the first time, a Bovine herpesvirus type 4 (BoHV-4)-based viral vector (BoHV4-∆TK-CCHFV-N) and its immunogenicity in BALB/c and protection potential in IFNα/β/γR-/- mice models in comparison with two routinely used vaccine platforms, namely, Adenovirus type 5 and a DNA vector (pCDNA3.1 myc/His A), expressing the same antigen. All vaccine constructs successfully elicited significantly elevated cytokine levels and specific antibody responses in immunized BALB/c and IFNα/β/γR-/- mice. However, despite highly specific antibody responses in both animal models, the antibodies produced were unable to neutralize the virus in vitro. In the challenge experiment, only the BoHV4-∆TK-CCHFV-N and Ad5-N constructs produced 100% protection against lethal doses of the CCHFV Ank-2 strain in IFNα/β/γR-/- mice. The delivery platforms could not be compared due to similar protection rates in IFNα/β/γR-/- mice. However, during the challenge experiment in the T cell and passive antibody transfer assay, BoHV4-∆TK-CCHFV-N was dominant, with a protection rate of 75% compared to others. In conclusion, vector-based CCHFV N protein expression constitutes an effective approach for vaccine development and BoHV-4 emerged as a strong alternative to previously used viral vectors. | tr_TR |
| dc.description.index | Pubmed | |
| dc.identifier.endpage | 20 | tr_TR |
| dc.identifier.issue | 3 | tr_TR |
| dc.identifier.startpage | 1 | tr_TR |
| dc.identifier.uri | https://doi.org/10.3390/v11030237 | tr_TR |
| dc.identifier.uri | http://hdl.handle.net/20.500.12575/72370 | |
| dc.identifier.volume | 11 | tr_TR |
| dc.language.iso | en | tr_TR |
| dc.relation.isversionof | 10.3390/v11030237 | tr_TR |
| dc.relation.journal | Viruses . | tr_TR |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | tr_TR |
| dc.subject | Crimean-Congo hemorrhagic fever | tr_TR |
| dc.subject | bovine herpesvirus type 4 | tr_TR |
| dc.subject | passive antibody transfer | tr_TR |
| dc.title | Bovine Herpesvirus Type 4 (BoHV-4) Vector Delivering Nucleocapsid Protein of Crimean-Congo Hemorrhagic Fever Virus Induces Comparable Protective Immunity against Lethal Challenge in IFNα/β/γR-/- Mice Models | tr_TR |
| dc.type | Article | tr_TR |