Physicochemical and Microbiological Characterization of Protected Designation of Origin Ezine Cheese: Assessment of Non-starter Lactic Acid Bacterial Diversity with Antimicrobial Activity

dc.contributor.authorAkçelik, Nefise
dc.contributor.departmentBiyoteknoloji Enstitüsütr_TR
dc.date.accessioned2020-10-30T08:57:48Z
dc.date.available2020-10-30T08:57:48Z
dc.date.issued2019
dc.description.abstractEzine cheese is a non-starter and long-ripened cheese produced in the Mount of Ida region of Çanakkale, Turkey, with a protected designation of origin status. Non-starter lactic acid bacteria (NSLAB) have a substantial effect on the quality and final sensorial characteristics of long-ripened cheeses. The dominance of NSLAB can be attributed to their high tolerance to the hostile environment in cheese during ripening relative to many other microbial groups and to its ability to inhibit undesired microorganisms. These qualities promote the microbiological stability of long-ripened cheeses. In this study, 144 samples were collected from three dairies during the ripening period of Ezine cheese. Physicochemical composition and NSLAB identification analyses were performed using both conventional and molecular methods. According to the results of a 16S rRNA gene sequence analysis, 13 different species belonging to seven genera were identified. Enterococcus faecium (38.42%) and E. faecalis (18.94%) were dominant species during the cheese manufacturing process, surviving 12 months of ripening together with Lactobacillus paracasei (13.68%) and Lb. plantarum (11.05%). The results indicate that NSLAB contributes to the microbiological stability of Ezine cheese over 12 months of ripening. The isolation of NSLAB with antimicrobial activity, potential bacteriocin producers, yielded defined collections of natural NSLAB isolates from Ezine cheese that can be used to generate specific starter cultures for the production of Ezine cheese (PDO).tr_TR
dc.description.indexPubmed
dc.identifier.endpage819tr_TR
dc.identifier.issue5tr_TR
dc.identifier.startpage804tr_TR
dc.identifier.urihttps://doi.org/10.5851/kosfa.2019.e71.tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/72157
dc.identifier.volume39tr_TR
dc.language.isoentr_TR
dc.relation.isversionof10.5851/kosfa.2019.e71.tr_TR
dc.relation.journalFood Sci Anim Resour .tr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectEzine cheesetr_TR
dc.subjectantimicrobial activitytr_TR
dc.subjectlactic acid bacteriatr_TR
dc.titlePhysicochemical and Microbiological Characterization of Protected Designation of Origin Ezine Cheese: Assessment of Non-starter Lactic Acid Bacterial Diversity with Antimicrobial Activitytr_TR
dc.typeArticletr_TR

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