Co-delivery effect of CD24 on the immunogenicity and lethal challenge protection of a DNA vector expressing nucleocapsid protein of crimean congo hemorrhagic fever virus

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dc.contributor.authorYılmaz, Erkan
dc.contributor.authorIDhttp://orcid.org/0000-0001-7355-3878tr_TR
dc.contributor.departmentDil ve Tarih-Coğrafya Fakültesitr_TR
dc.contributor.otherFarzani, Touraj Aligholipour
dc.contributor.otherHanifehnezhad, Alireza
dc.contributor.otherFöldes, Katalin
dc.contributor.otherErgünay, Koray
dc.date.accessioned2020-05-08T12:29:12Z
dc.date.available2020-05-08T12:29:12Z
dc.date.issued2019
dc.description.abstractCrimean Congo hemorrhagic fever virus (CCHFV) is the causative agent of a globally-spread tick-borne zoonotic infection, with an eminent risk of fatal human disease. The imminent public health threat posed by the disseminated virus activity and lack of an approved therapeutic make CCHFV an urgent target for vaccine development. We described the construction of a DNA vector expressing a nucleocapsid protein (N) of CCHFV (pV-N13), and investigated its potential to stimulate the cytokine and total/specific antibody responses in BALB/c and a challenge experiment in IFNAR −/− mice. Because of a lack of sufficient antibody stimulation towards the N protein, we have selected cluster of differentiation 24 (CD24) protein as a potential adjuvant, which has a proliferative effect on B and T cells. Overall, our N expressing construct, when administered solely or in combination with the pCD24 vector, elicited significant cellular and humoral responses in BALB/c, despite variations in the particular cytokines and total antibodies. However, the stimulated antibodies produced as a result of the N protein expression have shown no neutralizing ability in the virus neutralization assay. Furthermore, the challenge experiments revealed the protection potential of the N expressing construct in an IFNAR −/− mice model. The cytokine analysis in the IFNAR −/− mice showed an elevation in the IL-6 and TNF-alpha levels. In conclusion, we have shown that targeting the S segment of CCHFV can be considered for a practical way to develop a vaccine against this virus, because of its ability to induce an immune response, which leads to protection in the challenge assays in the interferon (IFN)-gamma defective mice models. Moreover, CD24 has a prominent immunologic effect when it co-delivers with a suitable foreign gene expressing vector. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.tr_TR
dc.description.indexPubmed
dc.identifier.endpage19tr_TR
dc.identifier.issue1tr_TR
dc.identifier.other75tr_TR
dc.identifier.startpage01tr_TR
dc.identifier.urihttps://doi.org/10.3390/v11010075tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/71228
dc.identifier.volume11tr_TR
dc.language.isoentr_TR
dc.relation.isversionof10.3390/v11010075tr_TR
dc.relation.journalVirusestr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectCCHFVtr_TR
dc.subjectCD24tr_TR
dc.subjectCytokinestr_TR
dc.subjectIFNAR −/− micetr_TR
dc.subjectLethal Challengetr_TR
dc.subjectNucleocapsidtr_TR
dc.titleCo-delivery effect of CD24 on the immunogenicity and lethal challenge protection of a DNA vector expressing nucleocapsid protein of crimean congo hemorrhagic fever virustr_TR
dc.typeArticletr_TR

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