Investigation of Carbapenem resistance mechanisms in Klebsiella pneumoniae by using phenotypic tests and a molecular assay

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dc.contributor.authorUs, Ebru
dc.contributor.departmentTıp Fakültesitr_TR
dc.date.accessioned2020-02-26T06:46:29Z
dc.date.available2020-02-26T06:46:29Z
dc.date.issued2019
dc.description.abstractIntroduction: The aim of this study was to investigate the presence of carbapenemase production and carbapenem resistance mechanisms in 47 carbapenem resistant Klebsiella pneumoniaeisolates by phenotypic confirmatory tests and molecular assay.Methodology: Carbapenem resistance genes KPC, OXA-48 and NDM were investigated with the BD MAX CRE assay kit in the BD MAX real time PCR instrument. Modified Hodge test, MBL gradient strip test, D70C Carbapenemase Detection Set, Temocillin gradientstrip test methodswere used as phenotypic confirmatory tests. Clonal relationship between study isolates was investigated with pulsed-field gel electrophoresis.Results: Analysis with BD MAX CRE assay revealed OXA-48 positivity in 17 (36%) strains, NDM positivity in 6 (13%) strains and coexistence of OXA-48 + NDM positivity in 8 (17%) strains. In 16 (34%) strains, none of the KPC, OXA-48 and NDM genes were detected. While MHT was the most sensitive phenotypic confirmatory test, D70C disc set had not been considered as a useful tool to assist the search for carbapenemase production. Temocillin gradient test alone could not be considered as sufficient to detect the presence of OXA-48. PFGE analyses revealed that 23 of 31 carbapenemase producing strains were in three major PFGEgenotypes (A, B and C). Conclusions: This study revealed that carbapenem resistance observed in K. pneumoniaeisolates was mainly due to OXA-48 and NDM genes and the increase of carbapenem resistance among K. pneumoniaestrains in our hospital was due to the interhospital spread of especially 3 epidemic clonestr_TR
dc.description.indexPubmed
dc.identifier.endpage1000tr_TR
dc.identifier.issue11tr_TR
dc.identifier.startpage992tr_TR
dc.identifier.urihttps://doi.org/10.3855/jidc.10783tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/70007
dc.identifier.volume13tr_TR
dc.language.isoentr_TR
dc.relation.isversionof10.3855/jidc.10783tr_TR
dc.relation.journalThe Journal of Infection in Developing Countries (JIDC)tr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectcarbapenemasetr_TR
dc.subjectKlebsiella pneumoniaetr_TR
dc.subjectKPCtr_TR
dc.subjectNDMtr_TR
dc.subjectOXA-48tr_TR
dc.titleInvestigation of Carbapenem resistance mechanisms in Klebsiella pneumoniae by using phenotypic tests and a molecular assaytr_TR
dc.typeArticletr_TR

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