Gas chromatographic analysis of PS, PI, SPH and LPC fatty acids in red blood cell membrane of rat, rabbit, human and dog
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2003
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Abstract
Fatty acid analysis of red blood cell (RBC) membrane PS, PI, LPC and SPH were examined in
species like rat, human, dog and rabbit by GC. C16:0 and C18:0 were the prominent SFA (saturated fatty
acids) in species phospholipids studied. C16:0 was higher in LPC and SPH whereas C18:0 was higher in PS
and PI in all species and in dog LPC.
Although evenly spread in phospholipids, MUFA (monounsaturated fatty acid) in PS, PI and LPC seamed
to be higher than in SPH. Among MUFA C16:1 and C18:1 were the prominent ones in the species, C18:1
being considerably higher than C16:1. PUFAs (polyunsaturated fatty acids) were relatively high in PS and
PI compared to LPC and SPH in each species. Arachidonate was one of the most abundant fatty acids in
human, rat and dog. Rabbit possessed very low levels of arachidonate in each phospholipid compared to
that of other species phospholipids. By contrast linoleic acid was high in rabbit RBC membrane
phospholipids. While C18:2 , C20:4 and C22:6 were the major PUFAs of human RBC membrane
phospholipids studied, C18:2 and C20:4 were the major ones in the others. 22-Carbon fatty acids were
present in small amounts in most species. C22:6 was present in rabbit, rat and human RBC membrane
phospholipids but very little was present in dog.
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Keywords
fatty acids, gas chromatography, analysis, RBC membrane, PS, PI, LPC, SPH, rabbit, rat, human, dog