Browsing by Author "Heydari, Hajar"

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    Sorbicillinoid-Based Metabolites from a Sponge-Derived Fungus Trichoderma saturnisporum
    (2018) Heydari, Hajar; Eczacılık Fakültesi
    Antibacterial activity assessment and high performance liquid chromatography associated with nuclear magnetic resonance (HPLC/NMR) data revealed that the EtOAc extract of the fermented endophytic fungus Trichoderma saturnisporum DI-IA, obtained from the marine sponge Dictyonella incisa, contained conjugated olefinic metabolites with antibacterial activity. Chemical examination of the fungal strain resulted in the isolation of eight new sorbicillinoid-based compounds, namely saturnispols A⁻H (1⁻8). Their structures were determined on the basis of extensive spectroscopic analysis, including the experimental and calculated electronic circular dichroism (ECD) data for the configurational assignments. Saturnispol F exerted significant inhibition against a panel of bacteria strains including vancomycin-resistant enterococci (VRE) with a minimum inhibitory concentrations (MIC) ranging from 1.63 to 12.9 μg/mL, while saturnispol H showed selective effects against VRE and B. subtilis.
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    Türkiye'de yetişen bazı lathyrus taksonları üzerinde farmakognozik araştırmalar
    (Sağlık Bilimleri Fakültesi, 2015) Heydari, Hajar; Saltan, Hayriye Gülçin; Sağlık Bilimleri
    The aim of this study was phytochemically investigation of aerial part of five Lathyrus L. species which belong to the Leguminosae family, and to evaluate their antioxidant, antibacterial and anti-inflammatory activities. L. armenus and L. cilicicus are endemic to Turkey. Isolation of secondary metabolites, kaempferol-3-O-glucosyl (1-2) ramnoside, kaempferol-3-O-ramnoside, rhamnocitrin rhamninoside, rhamnetin rhamninoside ve rhamnasine rhamninoside from L. armenus was performed by means of various chromatographic methods. The structure of the obtained compound were elucidated using spectroscopic methods. The total phenolic and total flavonoid content of the methanolic extracts of five species were determined using Folin-Ciocalteu and AlCl3 methods, respectively. Total phenolic content of the extracts was ranged 150.63-452.19 μg/mg and the total flavonoid content was ranged 36.19-105.43 μg/mg. Methanolic extract of L. aureus had the highest phenolic content and metanolic extract of L. laxiflorus exhibited the highest flavonoit content. The n-hexane, chloroform, ethyl acetate, methanolic and water extract of five Lathyrus species were also investigated for their radical scavenging activity by DPPH methods. Water extract of L. aureus was showed the strongest activity. The n-hexane, chloroform, ethyl acetate, methanolic and water extract of five Lathyrus species were assayed for in vitro antibacterial activity against two Gram positive (Staphylococcus aureus and Bacillus subtilis), two Gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and one fungus (Candida albicans), using the dilution methods. The highest antibacterial activity was produced by ethyl acetate fractions. The n-hexane, chloroform, ethyl acetate, methanolic and water extract of five Lathyrus species were investigated for anti-inflammatory activity by HRBC method. The highest activity was produced by ethyl acetate and aqueous fractions.