Browsing by Author "Albayrak, Harun"

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    Karadeniz Bölgesinde koyunlarda Peste des Petits Ruminants (PPR) enfeksiyonunun epidemiyolojisi
    (Sağlık Bilimleri Enstitüsü, 2007) Albayrak, Harun; Alkan, Feray
    Karadeniz Bölgesinde Koyunlarda Peste des Petits Ruminants (PPR)Enfeksiyonunun EpidemiyolojisiBu çalısmada son yıllarda Karadeniz Bölgesinde koyunlarda PPR süpheli vakalarınsıklıkla bildirilmesi nedeniyle, sözkonusu bölgede enfeksiyonun durumununserolojik ve virolojik olarak arastırılması ile virus izolasyonu ve RT-PCRyöntemlerinin tanısal degerinin karsılastırmalı olarak degerlendirilmesigerçeklestirilmistir.Bu amaçla Orta ve Dogu Karadeniz Bölgesinde yer alan il ( Samsun, Sinop,Ordu, Giresun, Trabzon, Rize, Amasya, Tokat) ve ilçelerden klinik ve nekropsibulgularına göre süpheli olarak degerlendirilen 34 enfeksiyon odagı virolojikçalısmalar için örneklenmistir. PPRV antikorlarını saptamak için, 49 koyunculukisletmesinden toplam 892 kan serumu kullanılmıstır. Virus izolasyon çalısmalarındaise, 34 süpheli odaktan 57 koyun ve kuzuya ait 41 akciger, 31 dalak, 10 lenfyumrusu, 22 defibrine kan, 20 oral swap, 20 nasal swap ve 20 konjunktival swapolmak üzere toplam 164 materyal saglanmıstır.Kan serum örneklerinin PPR C-ELISA testi kontrolü sonucunda; seropozitiflikoranı illere göre (%3,5-38,2) degismekle birlikte, örneklenen populasyon için%14,9 olarak saptanmıstır.Vero hücre kültüründe virus izolasyonu yapılamamıs olmasına karsın, RT-PCRile 164 materyalden 26 adedinde PPRV nükleik asidi tespit edilmistir.Bu sonucagöre PPRV enfeksiyonu süpheli odakların %44,1 (15/34) ve bu odaklardanörneklenen hayvanların %31,5'inde PPRV enfeksiyonu tanısı konulmustur. RT-PCRtestinde nekropsi örnegi olarak sırasıyla lenf yumrusu, dalak ve akciger; klinik olarakenfekte canlı hayvanlardan saglanan sırasıyla nasal ve konjunktival swapörneklerinin yüksek tanısal degerde oldugu belirlenmistir.Elde edilen verilere göre, Orta ve Dogu Karadeniz Bölgesi'nde PPRVenfeksiyonunun yaygın düzeyde varlıgı, enfeksiyonun bölgedeki durumununyerlesim birimleri bazında cografi kosullar (iklim, v.b) ve yetistirme sekli gibifaktörlere baglı olarak degiskenlik gösterdigi belirlenmistir. Ayrıca RT-PCR testininPPRV enfeksiyonunun tanısında yüksek tanısal degerlilikte oldugu saptanmıstır.AbstractEpidemiology of PPR Virus Infection on Sheep in Black Sea RegionDue to frequent reports of suspected PPR cases, the cases of infection wereinvestigated serologically and virologically, the diagnostic values of virus isolationand RT-PCR methods were compared and assessed.In this study, suspected 34 infection focuses as a result of the clinical andnecropsy findings were sampled for virological investigation in 8 cities (Samsun,Sinop, Ordu, Giresun, Trabzon, Rize, Amasya, Tokat), including their towns in theMiddle and Eastern Black Sea Region. Serum samples were collected from 892sheep in 49 herd and tested for antibody prevalance. Forty one lungs, 31 spleens, 10lymph nodes, 22 non-coagulated blood, 20 oral swap, 20 nasal swap and 20conjunctival swap from 57 sheep in suspected 34 infection focuses were used forvirus isolation investigation. The total number was 164.As a result of PPR C-ELISA test of serum samples; Although the proportion ofseropositive animals vary according to the provinces (%3,5-38,2), the average figurewas %14,9 for the sampled population.Although virus isolation could not be established in Vero cell cultures, PPRVnucleic acid was detected by RT-PCR in 26 materials out of 164. According to thisresult, suspected focuses of PPRV infections and sampled animals from this focuseswere diagnosed PPRV infection %44,1 (15/34) and %31,5 (18/57), respectively.Necropsy materials such as lymph node, spleen and lung respectively, clinicalsamples such as nasal swap and conjunctival swap respectively had been determinedby RT-PCR as high diagnostic value.According to obtained data, PPRV infection was widespread in the Middle andEast Black Sea Region. The prevalence of the infection in the region vary inaccordance with the factors such as geographical conditions (climate, etc.,), themethod of breeding. In addition, RT-PCR test offers more reliable results in thediagnosis of PPRV infection
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    Whole genome molecular characterization of Infectious Pancreatic Necrosis Viruses isolated in Turkey
    (Ankara Üniversitesi, 2019-12-26) Durmaz, Yüksel; Albayrak, Harun; Veteriner Fakültesi
    Infectious pancreatic necrosis virus (IPNV; Birnaviridae, Aquabirnavirus) causes infectious pancreatic necrosis (IPN) in fish. IPN disease was first found in 2002 in Turkey. In this study, 10 IPNV isolates were isolated between 2005 and 2013 and propagated in RTG-2 cell cultures. RNAs obtained from cell lysates were used as template and VP1, VP2, VP3, VP4 and VP5 genes of the virus were amplified in full length. Sequence analyses of the genes were made. The obtained sequences were compared with international reference strains from GenBank and phylogenetic analyses and genogrouping of the viruses were conducted. Turkish isolates were found to show a genetic similarity of between 93.5% and 99.8% in terms of molecules. When segment A sequences were compared with each other, a similarity between 97.8% and 99.8% was found; when they were compared with international reference strains, they were found to have the highest similarity (99.3%) with France AJ622822 isolate and the lowest (65.7%) similarity with Canada NC001915 isolate. When segment B sequences of the isolates were compared with each other, a similarity between 93.5% and 99.5% was found; when they were compared with international reference strains, they were found to have the highest similarity (98.9%) with Canada M58757 isolate and the lowest (52.7%) similarity with Finland KY548519 strain. As a result of phylogenetic analyses conducted, Turkish isolates were found to be closely related with France, Spain and Iran strains in serotype A2 and genogroup 5 they were placed in.