The effect of melatonin in rats with uterine torsion on uterus contractions, and the levels of ADMA, SDMA, arginine, Hsp90, TLR4, and NF-κB

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dc.contributor.authorDoğan, Halef
dc.contributor.authorRişvanlı, Ali
dc.contributor.authorSaat, Nevzat
dc.contributor.authorGül, Hüseyin Fatih
dc.contributor.authorİlhan, Necip
dc.contributor.authorŞeker, İbrahim
dc.contributor.authorŞahna, Engin
dc.contributor.departmentVeteriner Fakültesitr_TR
dc.date.accessioned2021-10-06T13:49:00Z
dc.date.available2021-10-06T13:49:00Z
dc.date.issued2019-07-14
dc.description.abstractIn this study was aimed at reducing uterine damage and increasing fertility after uterine torsion in pregnant animals. With this aim, uterine torsion was experimentally formed in 35 rats that were between 18-19 days pregnant. The animals were randomly divided into five groups, and melatonin was administered prior to torsion, at the time of torsion, and detorsion (10 mg/kg/gün IP). Ovario-hysterectomy operation was performed on all animals on the first day following parturition. Subsequently, from the obtained uterus samples, determination of the levels of asymmetrical dimethyl arginin (ADMA), symmetrical dimethyl arginin (SDMA), and arginine was made using the high-performance liquid chromatography (HPLC) and levels of Heat shock protein 90 (Hsp90), TLR4 (Toll Like Receptor 4) and NFκB (Nuclear factor kappa-light-chain-enhancer of activated B cells) were measured using the Western blot technique. The contraction-relaxation responses of the myometrium were also determined in the organ baths. According to the results of the western blot, higher protein expressions than those of the control group were determined in the second, third, fourth, and fifth groups in Hsp90, TLR4, NF-κB. The lowest values of arginine and ADMA were found in Group 3, whilst the lowest SDMA value was determined in Group 1. It was determined that melatonin reduces tissue damage secondary to torsio uteri and, furthermore, that administration of this hormone at the time of torsion formation was more effective than its administration at the time of detorsion.tr_TR
dc.identifier.endpage272tr_TR
dc.identifier.issn/e-issn1308-2817
dc.identifier.issue3tr_TR
dc.identifier.startpage267tr_TR
dc.identifier.urihttps://doi.org/10.33988/auvfd.467062tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/75271
dc.identifier.volume66tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.33988/auvfd.467062tr_TR
dc.relation.journalAnkara Üniversitesi Veteriner Fakültesi Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectMelatonintr_TR
dc.subjectPregnancytr_TR
dc.subjectRatstr_TR
dc.titleThe effect of melatonin in rats with uterine torsion on uterus contractions, and the levels of ADMA, SDMA, arginine, Hsp90, TLR4, and NF-κBtr_TR
dc.typeArticletr_TR

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