Phylogenetic analysis of partial transmembrane protein gene of canine coronaviruses detected in Turkey

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dc.contributor.authorYoldar, Zeynep Akkutay
dc.contributor.authorKoç, B. Taylan
dc.contributor.authorOğuzoğlu, T. Çiğdem
dc.contributor.departmentVeteriner Fakültesitr_TR
dc.date.accessioned2021-10-08T08:17:11Z
dc.date.available2021-10-08T08:17:11Z
dc.date.issued2020-06-03
dc.description.abstractCanine coronaviruses (CCoVs), a member of the family Coronaviridae, are the causative agents of acute gastroenteritis and are genetically divided into two groups, CCoV type I and CCoV type II. The aim of this study was to detect and characterize CCoV strains in fecal samples from six dogs exhibited gastrointestinal system symptoms. To determine the presence of the CCoV RNA, samples were tested by the reverse transcription-polymerase chain reaction (RT-PCR) assay targeting the partial M gene and then sequenced. Among six samples tested, two were found positive for CCoV RNA. Phylogenetic analysis was performed by Maximum-Likelihood (ML) method and revealed that one of the obtained field sequences was classified into CCoV-I genotype; and the other positive sample grouped in CCoV-II genotype. Both genogroups demonstrated broad genetic diversity. Phylogenetic analysis of amino acid sequences shows that our CCoV field strains was closely related to Italy and Brazil strains and placed on different genogroup clades in the CCoV cluster. Sequence comparison of the partial M gene revealed nucleotide identity of 71–100% and 68–100% similarity among the 25 coronavirus strains. TR/Ccv2 (MK636864) and TR/Ccv6 (MK636865) obtained in this study demonstrated 78.5-97.5% and 71–99% nucleotide identity with other CCoV strains around the world respectively. The results of the study demonstrate, CCoV strains from different genogroups are circulating in Turkey and this is a report on the phylogenetic analysis of a CCoV in Turkey, which there is limited information.tr_TR
dc.identifier.endpage271tr_TR
dc.identifier.issn/e-issn1308-2817
dc.identifier.issue3tr_TR
dc.identifier.startpage265tr_TR
dc.identifier.urihttps://doi.org/10.33988/auvfd.619074tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/75337
dc.identifier.volume67tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.33988/auvfd.619074tr_TR
dc.relation.journalAnkara Üniversitesi Veteriner Fakültesi Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectCanine coronavirustr_TR
dc.subjectPCRtr_TR
dc.subjectDogtr_TR
dc.titlePhylogenetic analysis of partial transmembrane protein gene of canine coronaviruses detected in Turkeytr_TR
dc.typeArticletr_TR

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Cilt:67 Sayı:03 (2020)