Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn

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dc.contributor.authorTurgut, İlhan
dc.contributor.authorZararsız, Duran
dc.contributor.authorYanıkoğlu, Semra
dc.contributor.departmentZiraat Fakültesitr_TR
dc.date.accessioned2021-09-28T07:01:02Z
dc.date.available2021-09-28T07:01:02Z
dc.date.issued2019-03-13
dc.description.abstractThis research was conducted at the breeding station of the Turkish breeding company Agromar A.Ş in the city of Bursa in Turkey during the 2013 and 2014 growing seasons. Used from within the same heterotic group crossings, 7 donor materials were obtained during the 2012 winter season in the greenhouse. The inducer line RWK-76xRWS, provided by University of Hohenheim, Germany, was used for generating haploid seeds. The donor and inducer crossing was performed during the 2013 summer season. The haploid selection and chromosome doubling were performed during the 2014 summer season. Seven donors were used for haploid induction which name are DNR1, DNR2, DNR3, DNR4, DNR5, DNR6, DNR7 respectively, from each donor different amount of ear crosses were performed (DNR1:16 ears, DNR2:10 ears, DNR3:10 ears, DNR4:12 ears, DNR5:11 ears, DNR6:13 ears, DNR7:11 ears). According to the present study, the average induction rate found ranged from 7.1 to 12.8%, and the average seedling survival rate in the greenhouse after colchicine application ranged from 57.9 to 77.6%. After transplanting to the field, 78.3-92.6% of these plants survived. As a result of this research, the chromosome doubling rate ranged from 22.5 to 48.3% depending on the donor material. These result indicates that maternal haploid selection visually is easy. Haploid induction rate (HIR) changes from donor to donor, its mean genotype and environment is effective for HIR. Average chromosome doubling rate is lower than other researchers’ results, it is also effected by genotype and chromosome doubling methods. Zea mays; Haploid line; Double haploid line; In vivo inductiontr_TR
dc.identifier.endpage69tr_TR
dc.identifier.issn/e-issn2148-9297
dc.identifier.issue1tr_TR
dc.identifier.startpage62tr_TR
dc.identifier.urihttps://doi.org/10.15832/ankutbd.539000tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/74970
dc.identifier.volume25tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.15832/ankutbd.539000tr_TR
dc.relation.journalTarım Bilimleri Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectZea maystr_TR
dc.subjectHaploid linetr_TR
dc.subjectDouble haploid linetr_TR
dc.titleProduction of Double Haploid Plants Using In Vivo Haploid Techniques in Corntr_TR
dc.typeArticletr_TR

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Cilt:25 Sayı:01 (2019)