Bazı protein kaynağı yemlerin rumende in situ (naylon torba) ve in vitro yöntemler ile protein yıkılabilirlikleri arasındaki ilişkiler
Özet
This research was conducted to determine in situ and in vitro protein degradabilities and the relations between in situ and in vitro protein degradabilities of some protein supplements used commonly in ruminant nutrition. In situ (nylon bag) and in vitro (enzymatic) methods were applied by incubation times of 1 and 24 hours for in vitro and 2, 4, 8, 16, 24, 48 hours for nylon bag method for each feed. Streptomyces griseus bacterial enzyme (Sigma XXV Type SP 5 147) was used as synthetical enzyme in the application of the in vitro method. According to results of the research, considering the correlation between in situ and enzymatic degradabilities for cotton seed meal (CSM), no correlations were found between INS-E and INV (1,24), but, the correlations between INS (16, 24) and INV (1, 24) values were significant (P<0.01); for solvent extracted CSM, correlations between in situ and in vitro values were significant (for INS-E and INV24, P<0.05 and for the others PO.01) and for sun flower meal (SFM) correlations between in situ and in vitro values were found significant (P<0.00 1) As conclusion, although regression equations for estimation of in situ from in vitro were significant and the correlations between in situ and in vitro values were high, there were considirable differences between in situ and in vitro protein degradabilities. KEY WORDS : In situ, in vitro, enzymatic, protein, degradability, correlations.