Eczanelerde çalışan eczacı ve eczane personelinde staphylococcus aureus burun taşıyıcılığının saptanması, suşların metisilin direnci ve meca geni yönünden araştırılması

Abstract

The aim of this study pharmacists employed in pharmacies and pharmacy staff to determine the nasal carriage of Staphylococcus aureus, is investigated for the mecA gene of methicillin resistance and strains. The determination of methicillin resistance of the isolates to determine the mecA gene by PCR as the gold standard.The study pharmacists and pharmacy staff in the pharmacy, including 300 who work in Ankara. In our study, those who received the volunteer disclosure form given to age, gender, preferences, in the last year to apply for hospital antibiotic use within one month of the last treatment purposes, how many years working as pharmacy staf by asking questions in the form of received information. Two specimens for culture and PCR experiment were collected from the each nostril of pharmacists and pharmacy staff with sterile cotton swabs. As a result of the study of 300 people in 64% (%21.3) S. aureus was detected. This isolates 4 (%1.33) were MRSA. The determination of methicillin resistance of the isolates to determine the mecA gene by PCR as the gold standard. In our study, oxacillin and cefoxitin disk diffusion disk test and show the result in parallel with the study. MRSA isolates by PCR and rapid to be achieved in a reliable manner; the MecA gene as the gold standard for the detection of MRSA by PCR to search for and we conclude that routine laboratory applications.