Asma çeşit ve anaçlarında kuraklık ve tuz stresi toleransına yönelik mikrodizin analizleri ve stres ile ilgili transkriptomların tespiti
In order to identify the differences within the mechanisms of water deficiency and salt tolerance of grapevine cultivars and rootstocks in transcriptomic level, 120 mM salt and water deficiency stress in the form of water deficiency were applied to Cabernet Sauvignon, 5BB and 41B genotypes for 7 days gradually. Among the transcriptome analysis lots of common stress transcripts were determined within each of three genotypes other than the specific transcripts for cultivar and rootstocks. The transcript ratios specific to genotype were detected for water deficiency stress as % 19.4 for Cabernet Sauvignon, % 13.3 and % 1.1 for 5BB and 41B, respectively; for salt stress as % 19.4, % 43.2 and % 3.3 for Cabernet Sauvignon, 5BB and 41B, respectively. It was found that the transcript ratios which are common for all three genotypes are % 34.2 for water deficiency stress and % 13.4 for salt stress. The water deficient stress transcript ratio was found to be higher by % 1-5 in comparison to salt stress, apart from the fact that lots of functional categories for both stress within the cultivars and rootstocks behave in parallel. Metabolism, protein metabolism and cellular transport categories were determined to be the most stress affected categories. It was determined that; when the transcription factors transcripts ((NAC domain, Myb-related transcription factors), the metabolite transcripts (1-pyrolin-5-carboxylase synthetase, arginine decarboxylase) and the hormonal related transcripts (ABA, Auxin and Ethylene) were induced by stress within the cultivars and rootstocks and it was found that the transcript ratios show different but important behaviors differences, dependent on stress type.