Kurşunun toksikokinetiğinde rol oynayan vitamin D reseptör gen polimorfizminin plasenta kurşun düzeylerine etkisi
Abstract
Although placenta has a function to decrease passing of harmfull substances, lead can cross plasental barrier easily with passive diffision and lead which enter the placenta accumulates until moment of birth. The aim of thi study is to determine the polymorphism of VDR FokI promoter region in pregnant women and investigate whether this polymorphism has an effect on lead levels in the placenta. The study population consists of 116 cases attending the Gynecolgy Department of Ankara University Faculty of Medicine. C→T single nucleotide polymorphism (SNP) was investigated in the womens' VDR FokI promoter region. The VDR FokI promoter region of the isolated DNAs were amplified with the PCR technique, the amplified oligonucleotides were cut with FokI restriction enzyme and typing was done. This thesis is the first study examining the effect of maternal VDR FokI polymorphism on lead levels in placenta. For the VDR C→T single nucleotid polymorphism on VDR FokI promoter region, the plasental lead level of the pregnants with ff homozygoust genotypes is found higher than the pregnants with FF homozygous and Ff heterozigous genotypes (p<0,05). The itamin D Receptor gene FokI polymorphism has an effect on lead levels in the placenta and the detection of higher levels of lead in placental tissue of mothers with ff genotype proposed that these mothers may expose their fetuses to increased lead-associated health risks. This study is intended to shed light on future work to be done on this subject