Türkiye'de (İç Anadolu) Y kromozomu polimorfizmi
Özet
ABSTRACT MASTER THESIS THE Y CHROMOSOME POLYMORPHISM IN TURKEY (CENTRAL ANATOLIA) Aydın RÜSTEMOV Ankara University Graduate School of Natural and Applied Sciences Department of Biology Supervisor: Prof. Dr. Ahmet KADIKIRAN Determination of frequency and distribution of human Y-chromosome short tandem repeats (STR) allelles and haplotype analysis plays an important role in fornesic medicine, paternity testing and tracing back to genomic drift in human evolution. Though disadvantages due to it's sex-limited nature, Y- STRs also have some advantages since they present as single copy sequences in genome and do not necessiate complex molecular methods which differs from autosomal STRs. In this study, eight Y-STR loci including DYS19, DYS388, DYS389/I, DYS3389/II, DYS390, DYS391, DYS392, DYS393 in males from Internal Anatolian Region were investigated. Normal healthy males living in the same geographical region for at least three generations were included in the study. PCR analysis were carried out with Y-STR specific primers on genomic DNA from peripheral blood samples after obtaining informed consent. Size determination of PCR products were detected by silver staining following %6 polyacrilamide gel electrophoresis (PAGE). Distribution of allelles for each Y-STR locus was determined and haplotype analysis was carried out and compared with data in the literature from other geographical regions. Regarding these eight loci, the lenght, repeat number and the frequencies of the most common alleles were found as 194(15) %56,1 in DYS19, 131(13) %66,1 in DYS388, 251(13) %48,15 in DYS389I, 371(30) %30,0 in DYS389II, 215(24) %33,96 in DYS390, 287(11) %52,0 in DYS391, 111254(13) %30,77 in DYS392 and 1 19(12) %49,12 in DYS393 respectively. Haplotype analyses was also performed in the content of this study. KEY WORDS: Y chromosome, STR, Microsatellite, Polymorphism, Haplotype, Turkey, Central Anatolia IV