Kontrollü ve kontrolsüz tip 2 diyabet hastalarında LDL'nin oksidasyona direncinin araştırılması
Özet
Diabetes Mellitus is a disease, which emerges due to severe insulin dysfunction, is characterized with lipid and protein metabolic disorders and is complex and frequently encountered. For this reason, several researches were conducted with regard to ethiopathogenesis. Lipid peroxidation plays an important role in the pathogenesis of diabetes mellitus as in the case of arteriosclerosis, cancer and many other diseases. Free radicals emerge as a result of long term hypoglycemia, increase of non-enzymatic glycosination of proteins in diabetes patients and the oxidation of these. Free radicals may attack cell membrane and components and eventually lead to the disorder of the organ. Aim: The aim behind planning this study was to research the LDL oxidation in the ethiopathogenesis of diabetes mellitus disease. Method: 90 Type 2 diabetes patients were grouped as per their HbAic values as controlled (< 5.7 %), weak (5.7 - 7.7 %) and non-controlled {>7.7 %). Further, a healthy control group of 37 has been included in the study for comparison with diabetes patients in terms of the sensitivity of LDL to oxidation and the antioxidant potential measurements. Heparin - citrate precipitation method was used in order to obtain LDL from the serums of patient and control group. Obtained LDL lipoproteins were exposed to oxidation with CuSCU and sensitivity to oxidation was evaluated with TBARS method. 10 controlled diabetes patients, 10 non-controlled diabetes patients and 10 healthy control patients were examined in terms of antioxidant potentials. 65Findings: Sensitivity to oxidation was found to be lower in weak controlled diabetes patients and in non-controlled diabetes patients when compared to the controlled group (p<0.05, p=0.01). No sound difference was found among groups when the antioxidant potential was studied; meanwhile compared to the controlled group the AOP was found to be low in both controlled and non-controlled diabetes groups. Conclusion: The conclusion reached by our study that "sensitivity to oxidation is lower in weak controlled diabetes patients and in non-controlled diabetes patients when compared to the controlled group" does not mean sensitivity to oxidation is better in diabetes patients. Antioxidant potential was found to be lower in diabetes groups compared to the controlled groups that are not meaningful statistically. This makes us think that oxidant stress increases in diabetes and oxidant defense systems weaken during the chronic course of the illness. Because of the decreased antioxidant potential, probably shortening the LDL oxidation lag phase, sensitivity to oxidation measurement was found to be lower in diabetes groups. Keywords: Diabetes, LDL Oxidation