Tunceli sarımsağında (Allium tuncelianum (Kollman, N.Özhatay, D. Matthew, Ş. Şiraneci) in vitro kök ve sürgün ucu kültürü yoluyla çoğaltma
Özet
Master ThesisPROPAGATION OF TUNCEL GARLIC ( Allium tuncelianum (KOLLMAN), N.ÖZHATAY,D. MATTHEW, Ş. Ş RANEC ) THROUGH IN VITRO ROOT AND SHOOT TIP CULTUREEzgi YAZARAnkara UniversityGraduate School of Natural and Applied SciencesDepartment of HorticultureSupervisor: Prof. Dr. Ruhsar YANMAZIn this research, possibilities for rapid propagation in shorter times for Tunceli garlic, which isendemic to our country, were determined by root culture and shoot tip culture methods whichare among rapid propagation and tissue culture techniques. Callus formation couldn?t beprovided in the roots in root tip culture studies. Although slight callus inception was observed inthe environment where there was 0.1 mg/1 BA and 1.0 mg/1 NAA, no advance was observedafterwards. Expected success was not achieved in the root tip culture study.In the shoot tip culture study, shoot formation started approximately 1 month after shoot tipplanting. After 4 sub-cultures, it was determined that environments containing IAA gave outbetter results than environments containing NAA with regard to the number of shoots. Whenenvironments are compared with regard to shoot rates, the highest shoot rate was obtained fromthe environment containing 0.05 mg/l BA+0.1 mg/l IAA with 76%. This was followed byenvironments containing 0.1 mg/l BA, 0.1 mg/l BA+0.1 mg/l NAA with 67% and 0.05 mg/lBA+0.5 mg/l IAA with 65%, 0.05 mg/l BA with 56%. It was determined that the propagationco-efficient of this species is low and approximately 1 shoot can be obtained per shoot. Of theauxins, NAA was shown to be more efficient in bulb formation in shoots and bulb formationincreased along with the increase in the number of sub-cultures.2006, 42 pagesKey Words: Allium , in vitro, root tip culture, shoot tip culturei