Evcil köpeklerde Canine corona virus (CCoV)'ların moleküler epidemiyolojisi
Özet
In this study, enlucidating not only the prevalance of CCoV, important disease of dogs, but also molecular characterization of the viruses with M and S genes based RT-PCR and sequencing were aimed. During this study 100 dogs were sampled. Dogs were grouped according to the information collected from the questionnaires filled by the owners on age, breed, gender, housing, environment and vaccination status. These questionnaires revealed that CCoV infection was seen widespread in dog population (%40), diseases could be caused by virus regardless of clinical symptome, vaccination status, gender and housing (indoor /outdoor). Diarrhoea is the common clinical symptom of CCoV. The PCR results indicated that not only the M primers but also the S gene primers should be used in order to determine and genotyping of the CCoV. The sequence analysis pointed out the CCoV‟s (CCoV type 1 and 2) in Turkey, were genetically related to the other CCoV‟s worldwide. As a result we concluded that studies for determining molecular epidemiology of canine coronaviruses must be continuous; and these studies will contribute to the choice of commercial vaccines and help new studies for developing vaccines and contribute to healthy animal in several ways.