Ruminantlarda Listerial abortların tanısı için PCR tekniklerinin optimizasyonu
Özet
Ruminants play an important role in livestock breeding in our country. In ruminant breeding, abortion is one of the leading problems, and one of the most important abortifacient diseases in Turkey is undoubtedly listeriosis. The main pathogens among Listeria species that cause abortion are L. monocytogenes and L. ivanovii. In this study, development of a PCR technique for the detection of pathogenic Listeria species (Listeria monocytogenes, Listeria ivanovii) causing listerial abortions in ruminants was described. The determined detection limit from bacterial dilutions was only 3 CFU/25 µl, while the detection limit from the artificial contaminated tissue ranged between 75-750 CFU/25 µl. Results obtained with the multiplex-technique from foetal abomasal fluid, fetal liver, and cotyledon samples were found to be more sensitive in case of L. ivanovii rather then L. monocytogenes. Milk is known as a source of bacterial shedding, therefore milk samples were included for artificial contamination research and the results were of equal sensitivity and ideal for both pathogens. A multiplex-PCR technique for the detection of Listeria species causing abortion in ruminants was not found in the literature; therefore, this thesis is considered to be original.