Glutatyon (GSH)) düzeyinin plasentada araştırılması
Özet
Smoking during pregnancy or exposure to cigarette smoke not only damages health of the mother but also appears the complications related to pregnancy and causes serious health problems in newborns. Cigarette is one of the most important source of oxygen free radicals. Free oxygen radicals can be controlled in high rates by the antioxidants. There is a biochemical protection mechanism that includes enzyme and antioxidants in the plasenta and it prevents injury induced by reactive oxygen species. Glutathione is an essential part of protective mechanisms against oxidative stres and makes its protective role via protects cellular targets against oxidative and electrophilic chemicals.In this study, it is aimed to investigate the relationship between cigarette and oxidative stres to compare results the placental glutathione rates which measures in 8 smoked pregnant women, 9 passive smoked pregnant women and 22 non smoked pregnant women placentas.Spectrophotometric method for determination of placental glutathione levels and fluorescence detector HPLC method for determination of a 1-hydroxy pyrene in urine were used. The HPLC methods validation were made in 0.2-30 nM concentration range for the 1- OHP. It was demonstrated that method was in a linear calibration range (r2 = 0.99496). It was studied with 3 different levels of standard spiked samples (0.2, 2, 30 nM) to prove interday repeatability, intra-day repeatability and recovery. The results of RSD (%) were respectively 8.73, 2.53, 1.19 for inter-day; 3.64, 1.19, 0.29 for intra-day; % 66.56, 85.01,92.3 for recovery. The method detection limit (LOD) and the lower limit (LOQ) were determined respectively 0.06 nM and 0.2 nM. 1-OHP analysis results were adjusted with creatinine levels and the results were calculated in ?mol / mol creatinine.In this study first of all it was compared that non-smoking pregnant women and smoked pregnant women and than smoke exposure of pregnant women and smoked pregnant women. It was meant that non-smoking pregnant women and smoke exposure pregnant women placental GSH levels significantly increased and urinary 1-OHP levels significantly decreased (p <0.05) to smoked women. Between urinary 1-OHP levels and placental GSH levels were not statistically significant correlation in smoked pregnant women (r = -0.27, p> 0.05).As a result, it was shown that oxidative stress were related with cigarette in pregnant women via measured by placental GSH level significantly decreased. It is considered that the results of this research will contribute the studies of the problems during pregnancy, such as pregnancy diabetes, preeclampsia, intrauterine growth restriction and preterm birth.