In vitro rekabetin keten (Linum usitatissimum L.) hipokotil eksplantlarından sürgün rejenerasyonu üzerine etkisi
Özet
This study was carried out to investigate the relationship between in vitro competition and stress and their effects on tissue culture response of hypocotyls of flax. Six weeks after culture initiation, hypocotyl fresh and dry weights, shoot regeneration percentage, shoot number per hypocotyl, shoot length and total shoot number per Petri dish were recorded. The results showed that encouraging explants for competition by decreasing the intervals among them increased shoot regeneration capacity till a certain point from where stress initiated and significant decreases in all parameters were observed. Explants cultured at 1.0 cm interval gave rise to the highest scores with respect to tissue culture response. Therefore, utilizing competition among explants in vitro may increase the success of tissue culture studies in other species.