Türkiye'nin kuzeyinde yayılış gösteren Yediuyur, Glis glis (Linnaeus, 1766) (Mammalia: Rodentia) populasyonlarının RAPD-PCR ile analizi
Özet
Edible dormouse, Glis glis (Linnaeus, 1766) (Mammalia: Rodentia) is small arboreal mammals living in dedicious, mixed forests, distributed in North of Turkey. However, because of the loss of habitat, gene flow between the populations of this species are interrupted. There are two subspecies of Glis glis in Turkey. Furthermore, the evolutional characteristics of this species and the importance of the isolation between Thrace and Anatolia population are not verified. There is no study at the level of DNA about the populations of Glis glis. The aim of this study is to contribute taxonomy and evolution of G. glis, analyzing its genetic structure. The Turkish populations of Glis glis is analyzed for the first time by using RAPD-PCR technique with this study. This study is conducted on 40 individual collected from the 7 localities of Turkey. 12 of the 20 RAPD markers tested yielded 74 polymorphic DNA bands. To show the genetic relation between the studied populations, genetic distance and similarity calculations of Nei were used. These calculations were done by using POPGENE software. The total genetic diversity and genetic differentiation values were calculated as Ht= 0,1480 and. GST = 0,6065, respectively. Dendrogram constructed based on genetic distance data formed two clusters, first cluster included in Kırklareli and Zonguldak populations. Second cluster was divided into two subgroups, first subgroup contained Ordu and Giresun populations, and second subgroup was formed from Trabzon, Rize and Artvin populations. When the Thrace and Anatolia populations are compared, genetic similarity value was found as 0,9319.