Türkiye'de halofilik arkebakterilerin izolasyonu ve karekterizasyonu
Özet
. Thesis ISOLATION AND CHARACTERIZAION OF HALOPHILIC ARCHAEBACTERIA FROM TURKEY Birgül ÖZCAN Ankara Universty Graduate School of Natural and Applied Sciences Department of Biology Supervisor: Prof. Dr. Cumhur ÇÖKMÜŞ Ninetyfive halophilic archaeal isolates were obtained from saline soil, brine and halit samples collected from six distinct regions of Turkey by using complex medium containing 25% NaCl (w/v). The isolates identified as archae by antibiotic suscebtibility and membrane ether-lipid analysis, were tested for motility, Gram reaction, pigmentation, cell and colony morphology, biochemical reactions, electrophoretic protein profiles and plasmid contents. The results indicated that 83,2% of the isolates were motile, 96.8% were Gram (-), 62% were rod shaped and 19% were in pleomorphic cell morphology. The most of the isolates had circular, entire and convex shaped colony morphology and their pigmentation were observed from light pink to red color. As a result of biochemical tests all halophilic archaea isolates were determined to be catalase and oxidase-positive, and only 2 isolates had anaerobic growth ability on L-argine. To other biochemical tests, the positive reactions were observed in 2.1-85.3% range. According to the antibiotic susceptibility tests, all isolates were susceptible to bacitracin, novobiocine and rifampicine. It was established that the 60 halophilic archaeal isolates contained plasmids varied in the numbers from 1 to 6 and in different molecular sizes ranged from 1 to 36.9 kb. On the other hand, whole-cell protein profiles of all isolates were obtained by SDS-PAGE, and the similarity dendograme was constructed from protein profiles by means of UPGMA method using MVSP analyses programme. Standard strains were clustured in 8 groups, and ten of our isolates were placed in the same group with standard strains while others formed distinct groups. 2004, 128 pages Key Words: Halofilik archaebacteria, isolation, characterization, plasmids, SDS- PAGE, Turkey