Ankara ilinde yetiştirilen kabakgillerde görülen hıyar mozaik virüsü izolatlarının serolojik yöntemlerle karakterizasyonu
Özet
In this research had been carried out to determine the serological characterization of Cucumber mosaic cucmnovirus in Ankara province which is the most important viral disease of cucurbits. For this purpose, surveys were conducted in July, August, September (first 2 weeks) 2002 and 230 plant samples were taken from young leaves of cucurbits. Surveys were done to Ayaş, Beypazarı, Çubuk, Nallıhan, Haymana, Kalecik, Kazan, Polatlı, Sincan and Şereflikoçhisar where the main cucurbits growing areas of Ankara province and the isolates which showed virus like simptoms were obtained. Collected isolates were mechanically inoculated to indicator plants, in addition DAS- ELISA and double diffusion tests were performed against the virus with all isolates and CMV subgroups were determined as serologically. As a result of the research, 7 isolates which were collected from Nallıhan of 230 isolates were determined as infected with CMV Subgroup-I. No result was obtained with the other specimens. Key Words : Cucumber mosaic cucumovirus (CMV), DAS-ELISA, Agar-gel assay, Cucurbits, CMV subgroup-I