İnsektisitlere dirençli Myzus persicae (sulzer) (Hemiptera:Aphididae) popülasyonlarında asetilkolinesteraz geni ve sodyum kanalı geninde meydana gelen mutasyonların incelenmesi
Özet
The peach potato aphid, Myzus persicae (Sulzer), is an important pest species in Turkey. Extensive use of insecticides has led to the selection of resistance to most chemical classes including organophosphates, carbamates and pyrethroids. Resistance to organophosphates and pyrethroids is often the result of mutations in the acetylcholinesterase and para type sodium channel protein, respectively. In this study several populations of M. persicae were collected from three different regions of Turkey in order to characterize insecticide resistance mechanisms. A combination of biochemical, DNA-based diagnostics and bioassays was used to assess the presence of three insecticide resistance mechanisms: carboxylesterase activity (E4 or FE4), insensitive acetylcholinesterase and insensitive sodium channels (knockdown resistance, kdr). Molecular work includes determination of point mutations associated with MACE (modified acetylcholinesterase) and kdr phenotypes using restriction fragment length polymorphism analysis (PCR-RFLP). According to the data obtained from this study it has been determined that M. persicae populations became insensitive to carbamates, organophosphates and pirethroids which is associated with MACE and kdr resistance phenotypes. When the carboxylesterase activities, bioassays and molecular results analyzed together significant resistance hasn?t been detected in the populations from Ankara. In contrast a high resistance levels were detected in the populations from Antalya and Mersin.