Civcivlerde velojenik ve mezojenik newcastle hastalığı virusu ile oluşturulan deneysel enfeksiyonda patomorfolojik bulguların karşılaştırılması
Özet
This study aims to examine, on macroscopic and microscopic levels, clinical findings that develop in chicks orally and occularly infected with viserotrophic velogenic and mesogenic strains of Newcastle disease viruses, particularly on days 3, 5, 10, 20, 25 and 30 of the infection. It also aims to study the pathological changes observed following necropsy in tissues and organs of these animals that expired or were terminated by euthanasia. In VVND infected groups, depression, puffing up of feathers, swelling around the eyes, lack of appetite, sneezing, sticking the neck forward and frequent opening and closing of the beak, shaking in the head, paralysis, loss of balance during movement and tortikollis have been clinically observed. During necropsy, petechial hemorrhages particularly located in the anterior of the proventriculus have been found. Furthermore, it has been observed that areas in the intestine that were lined by a grey and white circle or those that had a mottled red-grey appearance were frequently noticeable from the serozal surface. As for the groups infected with the Roakin strain, none of the above clinical or macroscopic findings have been seen. Histopathologically, the following have been observed in VVND infected groups; necrotic-hemorrhagic inflammation in proventriculus and the intestine; demiyelination in the central nervous system; gliosis, chromatolizis, satellitosis, perivascular cell infiltrations, necrosis in the purkinje cells and nörons; passive hiperemia in the liver; focal interstitial nefritis in the kidneys; hemorrhagia and emphysema in the lungs; lymphositic cell infiltrations in the interstitial areas; depletion, hyperplasia, necrosis in the spleen, bursa Fabricius, BALT and secal tonsilles; focal, multifocal or diffuse lymphositic cell infiltrations in the trahea, heart and pancreas. In groups that were infected with the Mesogenic Roakin strain, similar lesions have been found with only milder severity.In conclusion, it has been observed that ocular infections may develop more acutely when compared with oral infections, and that even in partially immune birds, the clinical, macroscopic and microscopic findings of VVND are consistent with the basic findings of this disease, which is generally reported in birds.