Çocuklarda streptokokkus mutans' ın vertikal (Aile içi) ve horizontal (çevreden) geçişinin araştırılması
Özet
37 children aged between 4-5 years who initially started in 2 different kindergartens, 19 of which started in a private and 18 in a public kindergarten, their mothers and kindergarten staff were included in this study with the aim of evaluation of vertical and horizontal transmission of S. mutans in children. Families were informed about the procedures and study to be applied and their approval was obtained. Intraoral examination of mothers and children was made according to WHO criteria (dmf-t/ DMF-T) and detail anamnesis was received from the mothers of children, then the mothers and children were caused to fill the questionnaire form including knowledge and attitudes of them about oral and dental healthcare. In the first session 1 ml saliva samples received from children and mothers in the same hours of the morning were examined microbiologically in order to isolate and quantify S. mutans. Arbitrarily Primed Polymerase Chain Reaction/ AP-PCR analysis was held on the same samples in order to determine the genetic characteristics of isolated S. mutans. Saliva samples were received with the same methods and the microbiological examinations and AP-PCR evaluations were repeated in order to evaluate the horizontal transmission in the 5thmonth which is enough time for contamination and coincided with the end of the 1st semester of the education year. It was determined that there is no statistically significant difference between the groups (p>0,05) both in terms of DMF- T values of mothers and dmf- t values of children. There is a statistically positive correlation (p<0,05) between mother DMF- T and child dmf- t values for the Group 1, and there is no statistically significant difference (p>0,05) between mother DMF- T and child dmf- t values for the Group 2. It was observed that there is a statistically significant correlation (p<0,05, p<0,01) between mother DMF- T values and mother-child S. mutans similarity level, however there is no statistically significant correlation (p>0,05) between dmf- t values of children and mother-child S. mutans similarity level. It was evidenced that the S. mutans amounts of the saliva belonging to the children in Group 1 is statistically higher than Group 2 (p<0,05), there is no statistically significant difference between two groups in terms of mothers, there is a statistically positive correlation (p<0,05, p<0,01) between child saliva S. mutans amount and mother saliva S. mutans amount in both groups. It was determined that there is not a statistically significant correlation (p>0,05) either in intra-group or inter-group between saliva S. mutans amounts of mothers and children and mother-child S. mutans similarity level.It was seen that the average similarity level is 54,62± 15,60% for Group 1 and 56,47± 28,22% for Group 2, there is no statistically significant difference (p>0,05) between motherchild S. mutans similarity levels in both groups. S. mutans similarity level among children is 44,12% for Group 1 and 46,72% for Group 2, there is no statistically significant difference (p>0,05) between similarity levels of children with each other in both groups. It was determined that there is 100% similarity between the kindergarten staff (driver), and teacher one, and child 12 and child 13 in Group 1, there is 80% similarity between the kindergartenstaff (cook) and child 4 in Group 2, while 58% similarity was seen between the teacher and child 1, child 2, child 8, child 12 and child 17.