Türkiye'deki ekonomik öneme sahip su ürünlerinin DNA barkodlarının çıkarılması
Özet
In this study, DNA barcoding was used in genetic characterization of 114 commercially important aquatic species found in Turkish fresh water and marine environments. Effects of tissue sampling and storage conditions on DNA quantity and quality, efficiency of DNA barcoding approach in determination of intraspecific and interspecific genetic distance and effects of choosing proper model and method in construction of evolutionary relationship trees to results were evaluated comprehensively within the context of this study. 654 base pair long DNA barcodes were generated for 114 commercially important aquatic species belonging to 4 phyla, 7 classes, 30 orders and 58 families from Turkish fresh water and marine environment and submitted to international data bases as first DNA barcode records from Turkey. After analyzing separate data sets (Actinopterygii, Arthropoda, Chondrichthyes, Cnidaria and Mollusca), all of the species were characterized with a unique DNA barcode sequence and DNA barcoding approach was evaluated as an effective molecular tool in characterization of intraspecific and interspecific relationship among these species.